流行病学研究表明大气细颗粒物暴露是引发心血管系统疾病的主要环境因素；纳米毒理学研究也证实某些纳米粒子可通过吸入方式进入血流，参加全身血液循环；这些研究均提示纳米材料暴露对心血管系统存在潜在影响并可能诱发或加重心血管疾病。前期研究集中在CNT对呼吸系统和血液造成的直接损伤方面，对CNT通过影响肺部免疫毒性介导血液毒效应的间接毒性并没有做深入的研究。本项目提出"碳纳米管暴露致肺免疫毒性损伤介导凝血纤溶系统功能改变的间接毒性作用机制"。拟选择碳纳米管为研究对象，采用大鼠气管滴注染毒动物模型和细胞暴露反应模型，从分子、细胞、组织器官和整体水平研究其对凝血纤溶系统损伤的间接作用形式和特点，利用RT-PCR、Western-blot及反义核酸等分子生物学技术重点探讨其分子机制。研究成果可为深入开展纳米材料生物安全性特别对心血管系统的影响研究奠定一定的理论和技术基础。

Epidemiology studies showed that atmospheric fine particles are important environmental factors to cardiovascular diseases. Nanotoxicology studies also confirmed that some nanoparticles could enter into blood circulation by inhalation. All these studies indicated that nanomaterials exposure would have potential influence on cardiovascular system and it is possible induce or aggravate cardiovascular disease. In our previous research results, we found that the erythrosedimentation (ESR) and endothelial protein C recepter (EPCR) were increased with the dose of carbon nanotubes exposure, which indicated that carbon nanotubes exposure could induce disorder between cruor and anti-cruor blance. So we have a hypothesis that the effects of carbon nanotubes on blood coagulation and fibrolysis system mediated by immunotoxicity in lung. We will use intratrachea instillation and cell culture to imitate immunological reaction induced by single walled carbon nanotubes to observe the indirect damage to blood-fibrolysis and its mechanism by RT-PCR、 Western-blot and RNAi. The results will provid some theory and technology foundation for biology safety of nanomaterials.