由钙离子信号启动蛋白磷酸化是抗病信号传递的重要途径。硅诱导厚皮甜瓜果实抗病反应过程质膜钙离子信号以及与磷酸化相关蛋白均有调节，但参与此过程的钙依赖蛋白(CDPK)的种类、活性的调节以及在磷酸化修饰中作用机制尚不明了。本项目拟选用厚皮甜瓜为材料，通过硅结合钙离子通道阻断剂(LaCl3)、CDPK抑制剂(TBB)以及及病原物接种处理，采用透射电子显微镜(TEM)、实时荧光定量PCR(RT-PCR)、多维液相色谱串联质谱(LC-MS/MS)、GST pull down等手段，在分子和生化水平解析参与硅诱导厚皮甜瓜果实抗病反应过程的CDPK基因与蛋白类型，明确其参与蛋白质磷酸化修饰的作用机理，为揭示硅诱导厚皮甜瓜果实抗病信号的传递作用机理奠定基础，也为深入揭示诱抗机理及开发新型激发子提供理论依据。

Protein phosphorylation triggered by calcium ion signal is recognized as one crucial pathway to transfer the signal. Calcium signal and phosphorylated protein from plasma membrane involved in disease resistance induced by silicate in muskmelon fruit; however, the types of calcium-dependent protein kinase (CDPK), regulation of its activities, and function of phosphorylation modified by CDPK have not been understood during the course of induced resistance in melon fruit treated with silicate. In this project, muskmelon fruit are chosen as material, modern analyzed methods of TEM, RT-PCR, LC-MS/MS and GST pull down are employed to reveal types of gene and protein of CDPK implicated in disease resistance in muskmelon fruit elicited by silicate, Lanthanum chloride Ca2+ channel inhibit, 4,5,6,7-tetrabromobenzotriazole (TBB) CDPK block and challenged inoculation with pathogens in molecular and biochemical level, and to explain the mechanism of protein phosphorylation mediated by CDPK and the proteins from plasma membrane involved in disease resistance. The objects of this project are to provide theory basis of function of resistance signal transduction from plasma membrane in muskmelon fruit induced by silicate, to understand deeply mechanism of induce resistance and develop new elicitors.