Dickeya软腐菌是世界十大重要植物病原细菌之一，寄主范围广，菌系分化明显。由D. zeae引起的香蕉细菌性软腐病2009年在广东大规模爆发，之后相继在我国多个香蕉产区出现。目前对该病的研究极少，病原菌致病机理不明确。我们前期研究发现，多胺及寄主类似化合物对病原菌的运动性和致病性起着重要的调控作用。本项目拟在前期的研究基础上，对香蕉软腐菌MS2进行全基因组测序，明确多胺合成代谢及运输途径的关键作用组分，重点研究病原菌及寄主产生的多胺对病原菌重要致病因子和III型分泌系统的调控作用，及对细菌运动性的调控机制，以揭示多胺信号的传导途径，明确多胺在跨界通讯中的作用，为病害防控提供新的作用靶标。

Dickeya species is one of the top ten important bacterial phytopathogens in the world, with a wide host range and differentials in strain characteristics. Banana soft rot disease caused by D. zeae broke out in Guangzhou in 2009 and thereafter spread to many banana planting regions, and has been included in China's newly invasive dangerous diseases. Study about D. zeae banana strain is rare and its pathogenicity mechanisms are unclear. Our previous study revealed that polyamines and host similar compounds play an important role during mediating pathogen motility and virulence. In this study, we aim to sequence the whole genome of D. zeae strain MS2, identify the key component(s) in polyamine synthesis and transportation, study in focus on the role of polyamines from pathogen and host in regulation of virulence factor production, T3SS gene expression and cell motility. Results from this study will help to elucidate the signal transduction and regulation mechanisms of polyamines, unveil their role in the transkingdom signal communication, and provide new targets for development of disease control strategies.