海胆性腺（海胆黄）既是其营养储备场所又是繁殖器官，富含ω-3多不饱和脂肪酸（ω-3 PUFA）与海胆的营养价值及其繁殖生理活动密切相关。脂肪酸结合蛋白（FABP）在高等动物中在ω-3 PUFA合成代谢中发挥着重要作用，但其在棘皮动物中的功能和作用机制尚不明确。中间球海胆是我国北方重要海水养殖品种，对PUFA合成代谢机制的研究是提高海胆性腺品质、培育性状优良个体的必要基础。申请人前期工作中筛选出两种FABP，随着PUFA含量变化而差异表达。本项目拟在前期工作基础上，以中间球海胆为研究对象，综合利用分子生物学及细胞生物学等方法，进一步明确FABP与ω-3 PUFA的相关性，揭示FABP在海胆ω-3 PUFA合成代谢中的功能及调控的分子机制，为解读FABP在棘皮动物PUFA的合成代谢过程中的功能及调控机制提供参考，对棘皮动物绿色健康养殖具有重要理论和实际意义。

Sea urchin gonad (sea urchin roe) is not only a place for its nutritional reserve but also a reproductive organ. It is rich in omega-3 polyunsaturated fatty acid (ω-3 PUFA), which is closely related to the nutritional value and reproductive physiological activities of sea urchin. Fatty acid binding protein (FABP) play an important role in the biosynthesis and metabolism of ω-3 PUFA in higher animals, but their function and mechanism in echinoderms are unclear. Strongylocentrotus intermedius is an important aquaculture species in the north of China. Research on the mechanism of PUFA biosynthesis and metabolism is an essential basis for improving sea urchin gonadal quality and breeding excellent individuals. In our pervious study, there were two types of FABP in sea urchin, and they were differentially expressed as the concentration of PUFA changed. Based on the previous work, we use S. intermedius as the research object to further clarify the correlation between FABP and ω-3 PUFA, and reveal the function of FABP and molecular mechanism of regulation in the biosynthesis and metabolism of ω-3 PUFA Moreover, it could provide a reference for understanding the function and regulatory mechanism of FABP in the biosynthesis and metabolism process of PUFA in echinoderms, and there are important theoretical and practical significance for the healthy breeding of echinoderms.