鱼类作为变温动物对温度等环境因子十分敏感。在长期的环境适应过程中，同种鱼类的不同地理亚群间的生长、繁殖等表型易受到温度等生境因子的驱使而表现出差异。以往对于鱼类生长特性的研究较多集中于个体水平或个别群体上，对于空间尺度上多群体间的比较研究有待加强。另外，对于鱼类响应气候变暖的环境适应性、生长差异的关键功能基因及其代谢通路尚不十分明确。本项目拟以分布于亚热带、中温带和温带的5个拉氏鱥（Phoxinus lagowskii）群体为研究对象，基于各群体的年龄生长分析，比较不同环境温度下的生长差异，结合群体遗传分析，探究其环境适应性。对生长差异的群体间进行比较转录组分析，挖掘与适应环境温度、机体生长相关的关键功能基因及其代谢通路，并辅以梯度温度的养殖试验，验证并分析关键基因的表达模式，阐明拉氏鱥生长对于温度变化的响应机制，为鱼类的生境适应、机体生长和生态系统服务等研究提供科学支撑。

As ectotherms, fish are very sensitive to environmental factors such as temperature. In the process of long-term environmental adaptation, the growth, reproduction and other phenotypes of different geographical subpopulations of the same fish species are susceptible to differences in temperature and other habitat factors. Previous studies on the growth characteristics of fish have mainly focused on individual levels or single population, and the comparative study of multi-populations on spatial scale needs to be strengthened. In addition, the key functional genes and metabolic pathways of fish in response to climate warming are not yet clear. In this project, five Phoxinus lagowskii populations distributed in subtropical zone, middle temperate zone and temperate zone will be selected as research objects. Basing on the age and growth analysis of each population, we would compare the growth differences under different environmental temperatures, and explore its environmental adaptability by combining with population genetic analysis. Furthermore, we would conduct comparative transcriptome analysis among populations with growth differences to explore key functional genes and metabolic pathways related to body growth and adaptation to environmental temperature. We would also set up gradient temperature culture experiments to verify and analyze the expression patterns of key genes. So that, we could elucidate the response mechanism of the growth of Phoxinus lagowskii to temperature changes. The results of this project should provide scientific support for studies on habitat adaptation, organism growth and ecosystem services of fish.