群体感应调控土传青枯菌入侵作物根际过程中的毒力行为。前期研究证实，根际有益菌解淀粉芽孢杆菌产生的易挥发性有机物(VOCs)能有效抑制土传青枯菌的毒力和根际入侵过程，推测VOCs中含有某种关键组分具有群体感应淬灭功能，能高效抑制土传青枯菌群体感调控的毒力行为。为解析根际有益菌VOCs介导群体感应淬灭阻断土传病原菌根际入侵的作用机制，本研究拟以根际有益菌解淀粉芽孢杆菌T-5产生的VOCs和土传青枯菌为研究对象，利用近期构建的土传青枯菌群体感应/淬灭报告体系，筛选发挥群感淬灭作用的VOCs关键组分；通过荧光标记技术，探究该组分在阻控番茄土传青枯病和土传青枯菌入侵番茄根际的作用效果；综合利用生理生化、分子生物学和转录组学等手段，解析该组分干扰群体感应相关毒力行为的生理生化和基因调控机制；为根际有益菌VOCs在阻控土传病原菌根际入侵过程的机理研究和产品开发提供理论指导和技术支持。

The virulent behaviors of Ralstonia solanacearum are under the regulation of the quorum sensing system during rhizosphere infection. Previous studies showed shat Volatile organic compounds (VOCs) produced by Bacillus amyloliquefaciens T-5 prevent the infection of R. solanacearum via inhibiting quorum sensing, i.e. quorum quenching. However, it is still unclear that the key functional components in T-5 VOCs. In this study, we attempted to identify the key VOCs components of B. amyloliquefaciens T-5 interference with R. solanacearum via a report system of quorum sensing. In the combination of multiple research methods of physiology, biochemistry, molecular biology and transcriptomics, we investigate the effects and regulation mechanisms of the key VOCs component on quorum sensing mediated pathogenesis. This research will provide the theoretical guidance and technical support for the fundamental study and application practice of VOCs-mediated management in soil borne diseases.