一个独脚金内酯受体AtD14新互作蛋白调控植物分枝的机理研究

独脚金内酯（SL）是一类重要的植物激素，调控植物分枝等影响作物产量的重要性状。SL受体AtD14在细胞核中与MAX2及SMXL7互作调控分枝，但细胞核以外的SL信号事件鲜见报道。本项目前期工作筛选并鉴定到一个依赖于SL与AtD14在细胞质中互作的新蛋白DIP18，过表达DIP18使拟南芥分枝增多。拟通过一系列生化、遗传及生信手段，揭示DIP18与AtD14互作的生理功能和生化意义，明确DIP18与AtD14的遗传关系，从而阐明DIP18在SL-AtD14信号网络调控植物分枝过程中的作用机理。本项目的成功实施，将发现可与AtD14在细胞质中互作的新蛋白，并揭示AtD14通过与DIP18互作调控植物分枝的分子机理，为研究AtD14同时定位于细胞核及细胞质的生物学意义提供线索，为SL信号转导研究打开新的视野，也将为通过分子设计育种改造植物株型来提高农作物产量提供新的基因资源和理论借鉴。

Strigolactone (SL) is an important class of plant hormones, regulating plant branches and other important traits that affect crop yield. SL receptor AtD14 interacts with MAX2 and SMXL7 to regulate branching in the nucleus, but SL signaling events outside the nucleus are rarely reported. Recently, we have screened and identified a new protein DIP18 that interacts with AtD14 in the cytoplasm though an SL-dependent manner. Overexpressing DIP18 can increase the primary branching number of Arabidopsis. This project is aimed to reveal the genetic relationship, physiological function and biochemical significance of the interaction between DIP18 and AtD14, during the SL-regulated plant branching. The successful completion of this project will identify a new protein that can interact with AtD14 in the cytoplasm, and reveal the molecular mechanism underlying the role of DIP18-AtD14 interaction in regulating plant branching. Our findings will open up a new window for studying SL signal transduction, and also provide new genetic resource and theoretical guidance for plant architecture and yield enhancement.