基于中药组方"谱-效"关系和蛋白芯片技术研究过敏康调节Th17/Treg亚群改善过敏体质的机制

过敏体质是多种过敏性疾病产生的背景与土壤。前期以中药组方"过敏康"作为主方干预过敏体质收到了良好的效果，并对组方进行了体外指纹图谱分析。本研究根据新近提出的中药复方"谱-效"关系理念，结合现代免疫学对Th17/Treg细胞亚群的最新认识，提出假说："过敏康"通过有效成分谱调控Th17/Treg亚群细胞因子及其信号通路的整体功能是改善过敏体质的重要机制。据此，拟采用信息融合技术，建立"过敏康"体外指纹图谱与体内血清药物化学相结合的HPLC-DAD和HPLC-ELSD指纹图谱；并采用蛋白芯片技术检测"过敏康"干预前后过敏体质人群Th17/Treg亚群细胞因子及信号通路的整体变化。整合以上研究结果，建立"过敏康"调节Th17/Treg亚群的"谱-效"关系网络，并对网络调控节点进行验证，旨在科学阐释该组方改善过敏体质的有效靶点和作用机制，为临床合理用药提供依据，对于指导过敏性疾病防治具有重要意义。

Allergic constitution is the background and soil of varieties of allergic diseases. Preliminary studies revealed that traditional Chinese medicine Guo Min Kang has good effect on allergic constitution, and fingerprints of the recipe in vitro had been analyzed. According to the newly proposed idea of TCM compound "spectrum-effect" relationship, combining latest modern immunology knowledge of Th17/Treg cell subsets, the following hypothesis is put forward, "One of the important mechanisms of Guo Min Kang to improve allergic constitution is through regulating cytokines of Th17/Treg cell subsets and the whole function of the signal pathway with the effective component". Based on these, this study will combine fingerprint results in vitro of Guo Min Kang and in vivo serum medicinal chemistry, establish the fingerprint of HPLC-DAD and HPLC-ELSD which can reflect its effectiveness. At the same time, use protein chip technology to detect allergic constitution people`s cytokines and signal pathways changes of Th17/Treg before and after the intervention of Guo Min Kang. Summarizing the results above, to establish spectrum-effect relationship network of Th17/Treg subsets regulated by Guo Min Kang, and verify the network nodes, scientifically interpret the targets and mechanisms of improvement of allergic constitution by Guo Min Kang, provide the basis for the clinical use of medicine to improve allergic constitution. This is of great significance to guide the prevention and control of allergic diseases.