等离子体生物医学应用已发展为一门独立学科，定量研究等离子体中的活性成分在生物组织中的穿透深度及其传递机制是等离子体临床应用急需解决的问题。本项目建立等离子体直接作用于生物组织的液相活性粒子的诊断模型。首先，采用最简单的液相环境——水，通过测量组织切片下水中活性粒子的浓度确定活性粒子的穿透深度问题。其次，考虑到真实生物组织细胞间质的复杂性，拟将真实生物组织切片置于不同液体环境中，研究活性基团的穿透情况。另外，结合气相活性粒子诊断方式，通过调控放电参数，诊断气相、液相活性粒子的密度，从而建立活性粒子从气相到生物组织的传递机制，优化和调控活性粒子，使其在生物医学应用中精准可控。最后，通过在组织切片下方的液相环境中培养细菌、真菌，探究等离子体射流穿透生物组织后的杀菌效果与测量的活性基团浓度之间的关系。定量研究等离子体中的活性成分在生物组织中的传递机制为临床应用中精准控制等离子体剂量提供理论指导。

Plasma medicine has developed into an independent discipline, and quantitative research on the penetration depth and transmission mechanism of active components in plasma is an urgent problem in the clinical application of plasma. This project establishes the diagnostic model of plasma directly applied to the liquid phase active particles of biological tissue. Firstly, the most simple liquid phase environment, double-distilled water, will be used to determine the penetrating depth of the active particles by measuring the concentration of active particles in the water under the tissue section. Secondly, considering the complexity of real biological tissue cells, it is proposed to study the penetration of active groups in different liquid environments. In addition, combined with the gas phase active particles diagnosis way, by regulating the discharge parameters, respectively in the diagnosis of the gas phase, liquid phase density of active particles, thus establishing active particles from gas phase to transfer mechanism of biological tissue, optimization and regulation of active particles, make its precision controlled in biomedical applications. Finally, we will cultivate bacteria and fungi at the liquid environment through the tissue section and explore the plasma jet sterilization effect in order to study the relationship between sterilization and the concentration of active group. Quantitative research on the transfer mechanism of active ingredients in plasma in biological tissue provides theoretical guidance for precise control of plasma dose in clinical application.