山西省煤碳资源丰富，煤碳业和以煤碳为能源主导的工业十分发达。煤矸石作为煤碳开采加工过程中的固体废物，其带来的扬尘、土壤酸化、重金属污染等一系列生态环境问题亟待解决。煤矸石山区环境治理的基础和核心是生态恢复，分离自矿区的原生外生菌根真菌（ECMF）和深色有隔内生真菌（DSE），在矿区植被生态恢复中有着巨大的应用潜力。本项目拟针对煤矸石山区最主要危害之一重金属，对山西省煤矸石山区ECMF和DSE多样性进行调查，并初步建立山西省煤矸石山区ECMF和DSE种质资源库。筛选出重金属耐性优异和有利于植物在煤矸石基质生存的优势菌株，结合室内与野外试验对ECMF和DSE在煤矸石山区生态恢复中的潜力进行评价。将菌株、植物以及野外环境结合，探讨煤矸石山区ECMF和DSE重金属耐性机制，以期为我国煤矿区生态重建提供菌种、理论依据和技术支持。

Coal industry and coal-based industry are well developed in Shanxi province, where coal resource is abundant. As solid waste of coal mining, gangue introduced many environmental problems, like raising dust, soil acidification, heavy metal (HM) pollution. The core of the environmental management at mining area is ecological restoration. Ectomycorrhizal fungi (ECMF) and dark septate endophytes (DSE), especially collected from mining area, have tremendous application potential in ecological restoration here. This project intends to investigate species diversity of ECMF and DSE in gangue dumps of Shanxi province, select strains which have high resistance to HM, or advantages in plant surviving at mining area, and evaluate fungal application potential in ecological restoration at mining area. We try to put fungi, plant and environment together to discuss the mechanism in HM tolerance of ECMF and DSE, and aim to provide valuable strains, theories and technologies for ecological restoration of mining areas in China.