Impact of salt and exogenous AM inoculation on indigenous microbial community structure in the rhizosphere of dioecious plant, Populus cathayana.
Impact of salt and exogenous AM inoculation on indigenous microbial community structure in the rhizosphere of dioecious plant, Populus cathayana.
复制标题
盐和外源AM接种对雌雄异株植物青杨根际本土微生物群落结构的影响
DOI：
10.1038/s41598-021-97674-w
复制
发表时间：
2021-09-15
影响因子：
4.6
通讯作者：
Tang M
中科院分区：
文献类型：
--
作者：
Wu N;Li Z;Tang M
雌雄异株植物对非生物胁迫的生理生化反应具有性别特异性，可能导致性别失衡，如何利用微生物缓解这一状况目前尚不清楚。利用杨树生长地土壤，对不同性别青杨在盐胁迫和外源AM接种条件下的生长参数、根际土壤理化性质进行了测定。此外，还比较了盐胁迫下不同性别青杨根际土壤性别特异性微生物群落结构。为明确与盐度和AM共生相关的性别特异性微生物群落特征，采用qPCR和DGGE相结合的方法对微生物群落多样性进行了监测。盐胁迫对幼苗的生长、生物量和营养元素积累都有一定的抑制作用，尤其是对雌株的抑制作用更为明显。外源AM接种处理减轻了这些负面影响，尤其是在75 mM盐处理下。与盐效应相比，外源AM接种处理对两性土壤理化性质的影响更大。DGGE结果表明，盐胁迫对真菌丰富度有负影响，但对真菌Simpson多样性指数有正影响，而外源AM接种处理则相反。采用结构方程模型（SEM）分析了盐和外源AM接种处理与生物量积累和微生物群落的因果关系：盐和外源AM接种处理对土壤元素含量、土壤性质的影响较为复杂，导致其与生物量积累和微生物群落的关系不同。盐胁迫对青杨根际土壤理化性质和微生物群落结构有负面影响，而外源AM接种对多数土壤理化性质和微生物群落状况有正面影响。
The sex-specific physical and biochemical responses in dioecious plants to abiotic stresses could result in gender imbalance, and how to ease the current situation by microorganisms is still unclear. Using native soil where poplars were grown, growth parameters, soil physicochemical properties in the rhizosphere soil of different sexes of Populus cathayana exposed to salt stress and exogenous arbuscular mycorrhizal (AM) inoculation were tested. Besides, the sex-specific microbial community structures in the rhizosphere soil of different sexes of Populus cathayana were compared under salt stress. To identify the sex-specific microbial community characteristics related to salinity and AM symbiosis, a combined qPCR and DGGE method was used to monitor microbial community diversity. Seedlings suffered severe pressure by salt stress, reflected in limited growth, biomass, and nutrient element accumulation, especially on females. Exogenous AM inoculation treatment alleviated these negative effects, especially under salt treatment of 75 mM. Compared with salt effect, exogenous AM inoculation treatment showed a greater effect on soil physical–chemical properties of both sexes. Based on DGGE results, salt stress negatively affected fungal richness but positively affected fungal Simpson diversity index, while exogenous AM inoculation treatment showed the opposite effect. Structural equation modeling (SEM) was performed to show the causal relationships between salt and exogenous AM inoculation treatments with biomass accumulation and microbial community: salt and exogenous AM inoculation treatment showed complicated effects on elementary concentrations, soil properties, which resulted in different relationship with biomass accumulation and microbial community. Salt stress had a negative effect on soil properties and microbial community structure in the rhizosphere soil of P. cathayana, whereas exogenous AM inoculation showed positive impacts on most of the soil physical–chemical properties and microbial community status.
登录
查看更多内容
影响因子：
5.2
作者：
Itoh H;Navarro R;Takeshita K;Tago K;Hayatsu M;Hori T;Kikuchi Y
通讯作者：
Kikuchi Y
影响因子：
9.7
作者：
Krishnamoorthy, Ramasamy;Kim, Kiyoon;Sa, Tongmin
通讯作者：
Sa, Tongmin
影响因子：
4.8
作者：
Bharti, Nidhi;Barnawal, Deepti;Kalra, Alok
通讯作者：
Kalra, Alok
影响因子：
4.4
作者：
Casamayor, EO;Schäfer, H;Muyzer, G
通讯作者：
Muyzer, G
影响因子：
9.7
作者：
Chowdhury, Nasrin;Marschner, Petra;Burns, Richard G.
通讯作者：
Burns, Richard G.