口腔癌是世界第六大常见癌，而舌癌是最常见的口腔鳞状细胞癌，是导致患者死亡的重要原因。但目前的治疗方法不够理想，急需对舌癌细胞信号传导的复杂性有进一步的了解，以发现新的治疗靶点。.C-RAF激酶，通过与众多结合蛋白的相互作用，调控着包括肿瘤发生在内的诸多生理、病理过程。本研究以互作蛋白组学的方法，对C-RAF激酶的结合蛋白进行分析，发现AAA+超家族成员RUVBL1为其重要结合蛋白。通过对RUVBL1的表达进行干扰，研究其对ERK信号、细胞分化、转化、凋亡和DNA合成的影响，拟建立舌癌动物模型，并通过基因突变等方法深入研究RUVBL1对C-RAF调控机制。本研究将为阐明C-RAF激酶及其结合蛋白相互作用机制及在舌癌发生过程中的作用奠定基础，为阐释C-RAF激酶及其结合蛋白的致癌机理提供新的线索，从而为治疗与C-RAF激酶及结合蛋白相关疾病，特别是舌癌提供全新的靶标。

Tongue cancer is a high incidence cancer worldwile. And it is the most common oral cnacer. It is a significant disease which could cause death. The treatment methods of tongue caner are not so effective until now. It is highly necessitous to investigate the complexity of signalling network of tongue cancer cells and to identify new therapeutic targets. .C-RAF kinase is a crosstalking point in cell signaling network, it regulates many physiological and pathological processes including tumorigenesis through its binding proteins. We have identified RAF kinase interactomes systematically and as an important binding protein, RUVBL1 has been identified. We will examine ERK signaling, cell differentiation, transformation, apoptosis and DNA synthesis through multiple perturbations of RUVBL1. And further we will investigate the effect of RUVBL1 on animal model of tongue cancer by multi-target perturbations. Our research will make clear the relationship between C-RAF kinase and RUVBL1 and find their function in the carcinogenesis of tongue cancer. Our work will identify new drug targets for C-RAF kinase and RUVBL1 related diseases, especially tongue cancer.