摘要：内膜新生是血管在各种损伤因素刺激下发生的病理改变，是多种心血管疾病共有的病理过程。ADAM22是一种多功能跨膜蛋白，现有的研究表明其对神经细胞和肿瘤细胞的增殖和迁移有调控作用。但是其在心血管系统疾病中的作用国际上尚无报道。本项目拟运用平滑肌细胞特异性ADAM22基因敲除小鼠，建立颈动脉导丝损伤模型，系统评价ADAM22对血管内膜新生的影响，并通过培养原代血管平滑肌细胞，探讨ADAM22作用的分子机制，为进一步阐明血管内膜新生的机制和寻找内膜新生相关疾病的防治新靶点提供理论依据。

Neointima formation, a common pathological event characterized by the thickening of blood vessels, accounts for the pathogenesis of several severe cardiovascular diseases. Until now, the underlying mechanisms of neointima formation remains not fully understood. ADAM22, a multi-functional transmembrane protein, possesses potent regulatory capacity on the proliferation and migration of neurocytes and cancer cells. However, the impact of ADAM22 on the process of cardiovascular diseases is largely unknown. In the present study, smooth muscle cells specific ADAM22 knockout mice will be used and challenged by wire-induced carotid artery injury to evaluate the effects of ADAM22 on neointima formation; the primary vascular smooth muscle cells will be employed to explore the potential molecular mechanisms, which might provide novel insight into the the etiology of neointima formation and might represent a promising strategy for the treatment of related vascular diseases.