肌腱组织再生期待给出力学性能强、具有生物活性的仿生的组织工程支架，本课题利用静电纺纳米纱线制备出仿生组织细胞外基质的新型肌腱支架，并使之负载生长因子，有望促进组织的再生。首先是创制出连续纳米纱线制备设备，用此设备制备出丝素-P(LLA-CL)纳米纱线、PET纤维增强型维纳米纱线、PLGA纤维增强型维纳米纱线和乳液静电纺含生长因子微纳米纱线，检测纳米纱线的形态结构和力学性能。然后将此四种纱线针织成四种纳米纱肌腱支架，表征支架的形态结构、测试力学性能和体外可降解性，研究生长因子从支架中的缓释速率。将肌腱细胞在支架上培养，研究细胞在针织支架上的三维长入情况，及生长因子和支架结构对细胞增殖速率和肌腱相关基因表达的影响。最后将四种支架植入兔子的跟腱缺损（1.5cm），不同时间取出再生肌腱测定其力学强度和组织学形态，观察支架材料降解过程和肌腱组织再生过程，揭示纳米纱结构及生长因子对肌腱再生的促进作用。

There is still no idear scaffold for tendon tissue engineering, this project will develop a nanoyan tendon scaffold by electrospining which could biomimic the nanofibrous structure of Extrocellular Matrix and promote the tendon tissue regeneration. Firstly we will prepare four kinds of nanofiber yarns, that is, SF-P(LLA-CL) nanofiber yarn, PLGA-SF-P(LLA-CL) micro/nanofiber yarn, PET-SF-P(LLA-CL) micro/nanofiber yarn and growth factor loaded PLGA-SF-P(LLA-CL) micro/nanofiber yarn by electrospinning and emulsion electrospinning using the nanoyarn spinner instrument developed by our Lab. The morphology and mechanical properties of nanofiber yarn will also be investigated. The further step is to knit the four kinds of nanofiber yarns into four kinds of tendon scaffolds (knitted SF-P(LLA-CL) nanofiber yarn scaffold, knitted PLGA-SF-P(LLA-CL) micro/nanofiber yarn scaffold, knitted PET-SF-P(LLA-CL) micro/nanofiber yarn scaffold and knitted growth factor loaded PLGA-SF-P(LLA-CL) micro/nanofiber yarn). The structure morphology, mechanical properties and biodegradability of the three knitted scaffold will be tested. The tendon cells will be cultured on the three scaffolds to investigate how fast cells proliferate on different scaffolds, how cells infiltrate three dimensionally into the scaffolds, how the gene express on the scaffolds and how the growth factor influence the cell proliferation speed and gene （collagen I, collagen III, tenascin C）expression level. Growth factor (bFGF and IGF-1) release behavior from knitted growth factor loaded PLGA-SF-P(LLA-CL) micro/nanofiber yarn scaffold will also be investigated. Finally the four knitted scaffolds will be implanted into rabbit to replace the achilles tendon defect(1.5cm) to investigated the new tendon regeneration. At different implantation time the regenerated tendon will be used to test the tensile strength and H-E histology analysis. From the animal test to show that the nanoyarn structure and growth factor can promote the tendon tissue regeneration, PET can enhance the mechanical properties of regenerated tendon.