土壤有机碳矿化与土壤质量和温室气体排放密切相关，明确其对气候变化的反馈是应对未来气候变暖的重要手段。不同施肥土壤有机碳矿化不同，微生物起着重要作用。目前对不同施肥土壤有机碳矿化与温度变化的响应关系研究不够深入，微生物的调控机制还不明确。故本项目以长期定位施肥试验为平台，首先研究不同施肥土壤有机碳矿化对温度响应的差异，其次监测微生物群落和限速酶的变化，通过分析有机碳矿化与微生物群落特征、限速酶的对应关系，探寻调控有机碳矿化温度敏感的微生物学机制。同时，利用13C-DNA-SIP技术，鉴定参与外源碳矿化的微生物种群，研究其参与外源碳矿化温度响应的动态过程。最后，通过综合评价不同施肥制度土壤微生物群落、酶类及参与外源碳矿化的关键微生物种群特征变化对土壤有机碳矿化温度响应的贡献，揭示土壤有机碳矿化对温度响应的微生物驱动机制，为预测不同施肥土壤对气候变暖的反馈提供理论依据。

Understanding the mechanisms of soil carbon mineralization's feedbacks to climate changes is critical to project future climate warming because of its significant relationships with soil quality and Green House Gas emission. The temperature responses to soil carbon mineralization under different fertilization regimes are uncertain so far and little is known about the mechanisms of soil microbes' dynamics although they play crucial roles in regulating carbon mineralization. In order to answer these questions, we use a long-term fertilization experiment as platform, firstly to investigate temperature responses to soil carbon mineralization, secondly to study the effects of temperature on soil microbial community composition and rate-limiting enzymes to analysis the relationships between changes in microorganisms/enzymes and soil carbon mineralization kinetics under warming, and then find out the microbial dynamic mechanisms of regulating temperature sensitivity of soil carbon mineralization. Meanwhile, we want to identify the microorganisms which control the added 13C-labelled carbon mineralization processes using DNA stable isotope probing technology, and study the dynamic processes of the added carbon mineralization which they participant in. Finally, we try to find the roles of shifts in specific functional microbial groups and enzymes which control the soil carbon mineralization under long-term fertilizer regimes and their contributions to soil carbon mineralization under warming and to elucidate microbial mediated feedbacks to temperature changes, which is fundamental to understanding the responses of different fertilized soils to climate warming.