多肽改性聚多巴胺纳米材料的生物传感研究及其在阿尔茨海默病分析中的应用

脑重大疾病的早期诊断及干预是脑疾病治疗的关键，发展脑重大疾病早期诊断新技术，对提高国民健康具有重大意义。本项目拟通过将聚多巴胺纳米材料（PDA）可控制备、表面功能化及组装、分子特异性识别和活体在线分析技术等相结合，开展基于多肽—PDA相互作用的生物传感研究；利用多肽作为基元对PDA实现表面功能化并整合多肽特异性分子识别过程，构建高效生物荧光传感新机制、新方法，并用于分析大鼠阿尔兹海默症（AD）病理模型的脑活体系统，实现高灵敏特异性检测AD相关的多种标志物在脑活体系统及体液（如：脑脊液、血液）中的浓度水平和随病程发展的变化规律。项目将从PDA表面改性新策略、生物传感识别新机制、分析新方法研究入手，从与AD相关单一标志物的检测，到多重标志物交互关系的研究，再到通过活体分析研究病理机制和早期诊断的探索，从而为AD的深入研究和精准医疗提供科学的途径。

The key to the treatment of major brain diseases is their early diagnosis and intervention, and to develop new technologies for early diagnosing major brain diseases is significant to improve the national health. This project aims to combine the technologies, including controllable preparation of polydopamine nanomaterials (PDA), surface functionalization and assembling, specific molecular recognition and live on-line analysis, for studying biological sensing based on the interaction of peptide and PDA. By integrated the surface functionalized PDA with peptide and its specific molecular recognition process, the novel mechanisms and methods for effect biological fluorescent sensing will be developed to analyze the brain living system of rats’ model with Alzheimer's disease (AD) pathology. The goal of high sensitive and specific detection of AD-associated markers will be realized in such system and body fluids (e.g. cerebrospinal fluid, blood) in view of their concentrations and changes over the course of disease development. In the project, the research will be started from the study of novel principles for PDA’s surface functionalization, new mechanisms for biological sensing and updated assays, and continue to detect sole AD’s biomarker, investigate the interaction between multiple AD’s biomarkers, furthermore, study the disease mechanisms and explore the potential of early disease diagnosis by in vivo analysis, so that a scientific way will be provided to AD’s thorough research and precise medical treatment.