感染性骨缺损修复一直是临床医生面临的难题，采用骨组织工程方法修复倍受关注。课题组前期研究发现：Sr-CaS有促进骨缺损修复的优势；NBD多肽可防治炎症对成骨细胞分化的抑制且与其阻断NF-КB信号通路有关。针对感染性骨缺损病程较长，成骨活性不足，NBD多肽局部用药效率低下、难以持续抗炎问题，本研究拟合成NBD多肽载药缓释微球，采用三维打印技术将Sr-CaS与NBD多肽载药缓释微球混合，低温制备符合骨缺损形状个性化支架材料。采用FTIR、XRD、Micro-CT、生物力学和分子生物学等手段观察材料表征、药物缓释特性、异位抗炎成骨及生物力学特点。该研究将微球缓释技术与三维打印技术相结合，实现药物高量装载和缓慢释放，保持药物活性同时利用Sr-CaS优良生物活性提高复合支架促新骨再生能力，为感染性骨缺损个性化治疗，维持病灶局部长效抗炎同时实现骨质缺损修复提供新的思路。

Repairment for infective bone defect (IBD) is always the challenge clinically, and bone tissue engineering have been payed more attention in such area recently. In the previous study, we have proved that Sr-CaS has accelerated bone healing in repairment for bone defect. Moreover, inflammation inhibits osteoblast differentiation, NBD peptide as an agonist was able to effectively antagonize the inhibition of osteoblast differentiation through down-regulating NF-κB pathway. Based on that IBD has a long course and insufficient osteogenic activity, and local application of NBD peptide is inefficiency in continuous anti-inflammatory, we will design NBD peptide sustained-release microspheres, structure personalized scaffold material of Sr-CaS/ NBD peptide according with bone defect shape relied on Three-Dimensional Printing (3DP) at low temperatures in this project. We will investigate materials characterization, drug sustained-release characteristic, biomechanics, anti-inflammatory and osteogenic capacity of scaffold, according to FTIR, XRD, Micro-CT, histology, biomechanics and molecular biology. The study will integrate sustained-release microspheres and 3DP technology, achieve high volume load, slow release and activity maintenance of NBD peptide, take advantage of osteogenic capacity of Sr-CaS. It will provide scientific evidence for individual-based treatment of IBD with both lasting anti-inflammatory and bone defect repairment.