Davidiin具有良好的抗肿瘤应用前景，但口服后体内不稳定，生物利用度低。增加GI粘附和上皮细胞的转运是提高口服生物利用度的关键。纳米粒可通过与GI 表面位点的亲和成分结合，增加口服药物的转运。基于此，我们拟在前期研究的基础上制备靶向PECAM-1的Davidiin玉米醇溶蛋白纳米聚合物，促进药物GI粘附和跨上皮运输，提高化合物的生物利用度。该传递系统利用多克隆抗体的多表位特征，激发细胞粘附因子介导的特殊囊泡内化途径传递药物，不干扰渗透屏障和细胞流动性，明显区别于经典细胞转运路径。我们拟主要采用荧光标记、电子显微镜以及放射性同位素追踪考察pAb-NCs与细胞结合的专属性、经细胞粘附因子介导的内化和经胞吞作用的转运。明确该传递系统在口服吸收后的Davidiin生物利用度和组织分布，为该传递系统特性提供可靠依据。从而为提高口服药物的生物利用度提供一个有效的新途径。

The result of pharmacology study has shown that Davidiin submits a good anti-tumor activity. But there is obviously known that Davidiin has ellagitannin characteristics of degradation and low oral bioavailability by oral delivery. The key problems are to improve mucosal attachment and enhance GI permeability. Binding of nanocarriers to specific cell surface marker can induce transport into and/or across cell. Based on this, The pAb-(Davidiin-Zein NCs) will be prepared utilized PECAM-1 as a target on GI epithelial cells. This provides internalization of prototype polymer NCs within these cells and transcellular transport by enhancing targeting, uptake,and transport in hunman GI epithelial cell multi-targeted to epitopes of the same receptor. Binding of anti-PECAM-coated polymer NCs to PECAM-1 trggers cell adhesion molecule (CAM)-mediated endocytosis, a pathway distinct from classical transport without apparent alteration of the permeability barrier or cell viability. Fluorescence and electron microscopy, and radioisotope tracing will reveal that anti-PECAM NCs specifically bind to cells in culture, are internalized via CAM-mediated endocytosis, trafficked by transcytosis across cell monolayers without disrupting the permeability barrier or cell viability, and enabled transepithelial transport of polyphenolics. The data come from oral bioavailability and tissue distribution after oral administration of Davidiin in rats will provide more reliable basis. So a new potential way will be shown to improve oral drug bioavailability.