基于功能磁性纳米粒子的长白山景天科植物的品质评价

选择合适的前处理方法是获取植物代谢物中的生物活性小分子信息的途径之一。为减少植物代谢物中的糖干扰其品质评价和获取植物源生物活性小分子，基于配体垂钓、共价可逆和非共价作用，选择性的磁分离的思路，设计、合成、表征、评估具有选择吸附糖和生物活性小分子的功能磁性纳米粒子(功能基团是硼酸、离子液体、生物大分子)。采取超声波萃取-固相提取-功能磁性纳米粒子吸附一体化的预分离和富集手段，以长白山景天科植物作为研究对象，消除糖干扰，制备特征提取物，用核磁、质谱、色谱等进行多组分测定，分离并检测具有生物活性小分子。依据方法重现性，结合化学计量学，开展长白山景天科植物及商用品的品质评价，建立质量鉴定标准，提供一种中药产品安全检测的前处理的规范方法。本项目的实施将为功能磁性纳米粒子在复杂样品中的预分离和富集做探索研究，也为其它药用植物的可持续开发利用提供新的思路和技术途径。

One of efficient routes of bioactive molecule information in plant metabolites is achieved by selecting suitable pretreatment methods. To decreases the concentration of sugars that usually overlap with plant metabolite signals involved its quality and obtain bioactive molecule information from plant source, relying on ligand fishing, reversible covalent and non-covalent interaction, the selectivity of magnetic separation, design, synthesis, characterization and assess- ment of functional magnetic nanoparticles will be developed for sugars and bioactive molecule adsorption, functional group consist of boric acid, ionic liquid and biomacromolecule will be conjugated into the surface of magnetic nanoparticles. Enrichment and pre-separation of bioactive molecules for spectroscopy analysis is presented. Spectral-feature-signatures for identifying will be achieved with a three-step and integrative purification protocol using the Crassulaceae on Changbai Mountain as a model plant. Liquid-solid extraction protocol using ultrasonic bath will be used to obtain crude extracts. Solid phase extraction is the second purification step. Application of magnetic separation technology, functional magnetic nanoparticles served as the third step. Since sugars are adsorbed, the desired analytes in sufficient purities and amounts will be obtained. The determination of multiponents based on NMR, MS and chromatography will be presented; isolation and detection of bioactive molecule will be promoted. Judging by reproducibility of method, spectroscopy analysis and multivariate analysis techniques will be applied for the identification, discrimination, and quality analysis of plant materials and its commercial products from Crassulaceae. The information on quality evaluation standards associated with metabolites of Crassulaceae will be developed. One type of safety of traditional Chinese medicine on specification methodology of detection pretreatment will be provided. By operating this project, functional magnetic nanoparticles applied in complex samples for pre-separation and enrichment will be explorative investigated. The new ideas and technical way of the sustainable development for other medicinal plants will be produced.