钝叶瓦松和八宝景天的抗炎、降糖成分及其作用机制的研究

药用植物是复杂体系，在一定程度上阻碍了它的研究进展，而活性成分是可以简化这个复杂体系的切入口，是药用植物发挥药效作用的物质基础。前期利用模式生物果蝇发现，钝叶瓦松和八宝景天水提物能够提高多种炎症因子引起的肠道免疫、缓解高糖饮食诱导的胰岛素代谢紊乱现象。基于此，为了进一步明确其活性成分的药理作用机制，本项目对2种药材进行溶剂梯度萃取，利用色谱和波谱进行活性成分的鉴定；选用环氧合酶和α-葡萄糖苷酶功能化磁性材料，一步分离活性成分；筛选调节果蝇肠道免疫、肠道炎症、胰岛素代谢的活性成分，并阐明其调控机制；依据构效关系，对活性成分开展指纹认定。最终，阐明钝叶瓦松和八宝景天中非单细胞水平的抗炎、降糖的活性成分及其作用机制，为药用植物综合开发与利用提供理论基础。

Medicinal plants are complex systems, which hinder their research progress to a certain extent. Active components are the key to simplify this complex system, which are basis to medicinal plants to exert their pharmacological effects. Preliminary studies using model organisms Drosophila melanogaster showed that water extracts of Orostachys malacophylla and Hylotelephium erythrostictum could improve intestinal immunity induced by various inflammatory factors, alleviate insulin metabolic disorders induced by high-sugar diet. Based on preliminary medicinal data, in order to further clarify the pharmacological mechanism of their active components, in this project, two medicinal materials will be extracted by solvent gradient method and active components will be identified by chromatography and spectroscopy. The cyclooxygenase and α-glucosidase functional magnetic materials will be used to separate the active components in one step. Active components regulating intestinal immunity, intestinal inflammation and insulin metabolism of Drosophila melanogaster will be screened and their regulatory mechanisms will be elucidated. Fingerprint identification of active components will be developed, according to structure-activity relationship. Finally, the non-single cell level anti-inflammatory and hypoglycemic active components and their mechanisms in Orostachys malacophylla and Hylotelephium erythrostictum will be elucidated, thus, theoretical basis for the comprehensive development and utilization of medicinal plants will be provided.