前期研究表明，CO2浓度升高可通过改变寄主植物诱导抗性影响蚜虫种群动态，但其调控的分子机制并不清楚。本项目拟利用野外开顶式二氧化碳（CO2）浓度控制箱，以CO2浓度升高为处理因子，以烟草和烟蚜为研究对象，基于植物受到烟蚜为害后最先诱导的丝裂原活化蛋白激酶（MAPKs）信号通路入手，以转基因植物（irSIPK，irWIPK，irSIPK，irAOC，NahG）与野生型烟草为研究材料，通过分析下游植物抗性激素的合成与代谢、植物抗性基因的表达以及蚜虫种群适合度，系统研究MAPKs信号途径在烟蚜介导的烟草免疫防卫系统中的作用及其对CO2浓度升高的响应机制。通过比较RNAi转基因材料对蚜虫及CO2浓度升高的响应特征，了解烟蚜对不同RNAi转基因植物与野生型烟草的为害特征、取食行为及种群参数的变化规律，从而明确MAPKs信号途径在植物响应蚜虫为害过程中的作用，预测植物与蚜虫互作关系在未来CO2浓度升高

Previous studies found elevated CO2 could affect the population dynamic of aphids by altering the resistance of host plant, however, the mechanism is unknown. The field open-top chambers are used to control the doubled-ambient carbon dioxide (CO2) concentration for the treatment factor, the plant Nicotiana attenuata and tobacco aphid Myzus persicae as the research object, plant mitogen-activated protein kinases (MAPKs) signaling pathway as the starting point, defensive phytohormones, and stably transformed N. attenuata plants silenced in WIPK(irWIPK), SIPK(irSIPK), MPK4(irMPK4), AOC(irAOC), SA synthesis (NahG) and wild-type WT are comparatively examined in this project. From the defensive phytohormones, aphid-induced defense pathways, the tobacco aphid feeding behavior, population fitness and molecular regulation point of view, we comprehensively examine the function of MAPKs signaling pathway in the interaction between tobacco and aphid when response to elevated CO2. This project compares the response characteristics of irWIPK, irSIPK, irMPK4, irAOC, NahG plants with wild-type plants under elevated CO2, to prove the effect of MAPKs signaling pathway and elevated CO2 on the synthesis of defensive phytohormones (Jasmonic acid and salicylic acid) in host plant when infested by aphid. Pea aphid infestation characteristics and population parameters on irWIPK, irSIPK, irMPK4 and wild-type tobacco were determined, in order to clarify the role of MAPKs signaling pathway in plant response to aphid infestation, and eventually to reveal the response mechanisms of interaction between plant and herbivorous insect to elevated CO2. The outcomes of this project are able to predict the future development trends of the interactions between plants and aphids in the context of elevated CO2 concentration, in order to provide a scientific basis on giving a full play to the way to the main source of resistance against the crop pests in ecological pest control.