红茶改善秀丽线虫氧化应激能力的分子机理研究

氧化应激是细胞功能失常或特异分子信号转导引起机体的一系列生理、病理及适应性反应，它与许多慢性疾病密切相关。目前，抗氧化性能的研究已从清除体内自由基能力演变为从整体上评价其改善机体内源性抗氧化系统效能。申请者前期研究表明，红茶能显著延长胡桃醌诱导产生氧化应激条件下模式生物秀丽线虫的平均寿命。本项目拟在此基础上，以秀丽线虫为活体模型，进一步探讨不同环境应激条件下红茶对秀丽线虫寿命的影响；利用现代分离纯化技术与活性评价跟踪相结合的方法获得红茶改善氧化应激能力的功能因子；采用cDNA表达谱芯片和差异蛋白质组学技术深入研究红茶功能因子改善氧化应激作用网络通路；通过免疫印迹、实时荧光定量PCR和基因突变等技术对相关功能基因从细胞与分子水平进行验证，以探明红茶改善氧化应激能力的分子机理。本研究有助于进一步探明红茶对由氧化应激引起的相关慢性疾病的预防作用及机制，为红茶的健康消费与高效利用提供科学依据。

Oxidative stress triggers a range of physiological, pathological, and adaptive responses in cells either as a result of cellular damage or through specific signaling molecules.Antioxidant activity had been determined by the ability of compounds to scavenge free radicals of organisms in previous studies. Recent findings support that endogenous expression and regulation of antioxidant genes serve as a direct role in protection against oxidative stress. Owing to actually increasing the risk of chronic deseases, it is of important practical value to study oxidative stress. Our previous research showed that black tea significantly increased the mean lifespan of C. elegans under juglone-induced oxidative stress. On the basis of this work, The molecular mechanism of black tea protective responsing to oxidative stress in C. elegans will be achieved in this research as follows: Effect of black tea on the mean lifespan of C.elegans under different environmental stresses will be developed further. The functional compounds of black tea responsing to oxidative stress will be obtained by combining modern isolation and purification technologies with activity evaluation. The signal pathway networks will be systematically investigated by using the methods of cDNA expression profile chip and difference proteomics. The functional genes will be comfirmed at the cellular and molecular level by western-blotting,realtime quantitative PCR and genetic mutation. The work will give a beneficial support for studying the protective effect of black tea against chronic diseases associated with oxidative stress. It also will provide scientific basis for reasonable developing the health protection of black tea.