神经病理性痛是临床最为常见的慢性疼痛之一，目前仍缺乏有效的治疗措施。研究表明甘丙肽在脊髓和脑内均具有镇痛作用，但其作用机制不清。本研究拟通过构建大鼠神经病理性痛模型、谷氨酸刺激伏核原代神经元、NG108-15或PC12细胞系，结合离体和在体实验方法。研究:(1)甘丙肽在神经病理性痛大鼠伏核的镇痛作用；(2)检测甘丙肽受体（GalR1,GalR2,GalR3）mRNA以及神经病理性痛信号通路中的关键分子ERK，PKA，PKC和CaMKII的表达，探索伏核内神经病理性痛的信号通路；(3)伏核注射三型GalR激动剂后注射ERK或PKA或PKC或CaMKII的抑制剂，明确甘丙肽对神经病理性疼痛镇痛作用的具体信号机制。本研究可为临床研究神经病理性痛镇痛药物作用靶点及甘丙肽脑内镇痛作用机制的研究提供直接的实验依据，对神经病理性痛的临床治疗具有重要的指导意义。

Neurological pain is the most common kind of chronic pain in clinic,but there is lack of an apparent treatment.Some studies showed that galanin had important antinociceptive roles in the spinal cord and brain,but the underlying mechanism was not clear.This research aims to combine in vitro with in vivo experimental models including the use of the sciatic nerve injury rats and primary cultured neurons and NG108-15 or PC12 cell lines to study: (1) the antinociceptive roles of nucleus accumbens(NAc) injection of galanin in rats with neurological pain. (2) The expression of galanin receptor(GalR1,GalR2,GalR3) mRNA and ERK, PKA, PKC, CaMKII of signal pathways in neurological pain will be investigated. It will explore the involvement of signal pathways in neurological pain. (3) GalR1 or GalR2 or GalR3 agonist will be injected into the NAc of rats, and followed 5 min later by intra-NAc injection of inhibitors of ERK,PKA,PKC or CaMKII to determine which kind of the signal transduction pathways is involved when GalR is activated. This study provides a direct basis for researching the targets of analgesic drugs for neurological pain in clinic; and it provides direct experiment basis for studying the signal pathways of antinociceptive roles of galanin in the brain.It has important clinical significance for treatment of the neurological pain.