红花为活血化瘀常用药，但药材来源于油用和油药兼用品种，品质参差不齐。类黄酮是红花的主要活性成分，为红花品质评价的重要指标。申请人前期基于转录组数据全面筛选和鉴定了红花类黄酮合成功能基因，克隆并验证了转录因子CtFRMYB1调控红花类黄酮合成关键基因CtCHS的表达。本研究基于CtFRMYB1解析红花类黄酮调控机理，并以此为基础，开展与类黄酮关联的SNP功能标记研究，以期指导红花优质药用品种的筛选和分子辅助育种。研究采用染色质免疫共沉淀技术与二代测序相结合的ChIP-Seq技术及凝胶阻滞迁移实验，解析CtFRMYB1调控红花类黄酮合成机理；在构建的红花群体中开展CtFRMYB1基因多态性与类黄酮关联分析，筛选与总类黄酮及其主要活性成分相关联的SNP功能标记。此工作的成功开展不仅对阐明红花类黄酮合成的分子调控机理具有重要的学术意义，同时对红花优质药用品种的筛选和分子辅助育种具有重要的应用价值。

Safflower is wildly used in Traditional Chinese Medicine for blood circulation drug. The herbs of safflower usually come from oil or oil-medicine varieties with uneven quality. Flavonoids are the main active ingredient for blood circulation in safflower, which is an important indicator for the quality evaluation of safflower. In our previous study, the genes involved in the flavonoid biosynthesis in safflower were screened and identified based on the transcriptome data. The transcription factor CtFRMYB1 was cloned and proved to be involved in flavonoid biosynthesis by regulating the expression of the chalcone synthase gene CtCHS. In this study, based on transcription factor CtFRMYB1, we analyze the regulation mechanism of safflower flavonoid biosynthesis, and then develop flavonoid-associated SNP functional markers, in order to guide the selection and molecular assisted breeding of high quality medicinal varieties of safflower. ChIP-Seq and EMSA experiments were carried out to analyze the regulation mechanism of CtFRMYB1 on the flavonoid biosynthesis safflower in safflower. Association analysis of the flavonoids and the CtFRMYB1 polymorphism was carried out. This work not only has important application value for the screening and molecular assisted breeding of high-quality medicinal varieties of safflower, but also has an important academic significance for elucidating the molecular regulation mechanism of flavonoid biosynthesis.