利用杂草病原微生物代谢产物中的活性物质作为先导化合物开发微生物源除草剂是新型除草剂研究的重要方向，已显示出良好的应用开发潜力。多孢木霉（Trichoderma polysporum）是近来研究发现的对青海农田恶性杂草具有广谱、高效除草活性的一种重要杂草病原真菌。本研究拟通过多孢木霉培养条件优化、活性物质分离纯化、结构鉴定及除草机理研究，以期筛选出一种获取大量多孢木霉活性物质的培养方法，形成一套分离提取多孢木霉活性物质的技术体系，鉴定除草活性组分的化学结构，明确多孢木霉活性物质的除草机理，为将该菌进一步开发成微生物源除草剂，应用于生产实践提供理论依据。

Using active substance of weeds pathogenic microbial metabolites as lead compounds for the development of microbial herbicides is the important direction of new herbicides research,which has shown good development potential. Trichoderma polysporum was recently discovered as an important bilcontrol fungi agent which had high herbicidal activity against malignant weeds in Qinghai farmland. This study intends to explore the optimism culture condition of Trichoderma polysporum, isolation and purification of active substance, structural identification and herbicidal mechanism, so as to select a method for obtaining a large amount of Trichoderma active substance, form a technology system of separation and extraction of active substance of Trichoderma polysporum, identify the chemical structure of herbicidal activity components and clear herbicidal mechanism of active substance of Trichoderma polysporum. This study will benefit further development for Trichoderma polysporum into microbial herbicides, and provide a theoretical basis for production practice.