近年来土壤重金属铬污染严重威胁农业生产，利用植物进行重金属污染土壤修复成本相对较低，且具有良好的生态效益。中国芒(Miscanthus sinensis)具有较强的耐重金属铬特性，是修复污染土壤最有潜力的多年生作物之一，然而其耐重金属分子机制尚未完全明确。本项目拟在前期收集、评价大量中国芒资源的基础上，1）系统分析重金属铬胁迫对中国芒地上及地下部分的影响，筛选对重金属铬耐/敏感材料，利用高通量转录组深度测序技术(RNA-Seq)挖掘差异表达基因，进行网络共表达分析；2）构建关联作图群体，对关联群体进行全基因组GBS-SNP、SSR基因型鉴定及全基因组关联分析(GWAS)；3）联合RNA-Seq与GWAS挖掘调控主效基因，揭示中国芒耐重金属铬分子机制。研究结果可为今后中国芒耐重金属铬胁迫分子育种提供重要的材料及理论支撑。

In recent years, the increasing soil heavy metal pollution, including hexavalent chromium, has become a serious threat to agricultural production. The use of plants for heavy metal contaminated soil repair is cost effective and ecological benefits. Miscanthus sinensis is one of the most potential plants for heavy metal contaminated soil repair, with strong tolerance to heavy metal hexavalent chromium and abundant biomass. However, the molecular mechanisms of tolerance to heavy metals hexavalent chromium are not entirely clear. Based on the collection and evaluation for a large number of M. sinensis germplasm resources, the project is designed to: 1) conduct the phenotypic and genetic evaluation of M. sinensis to heavy metal chromium stress and identify differentially expressed genes based on RNA-seq, discovery the molecular mechanisms of tolerance to heavy metal hexavalent chromium stress in resistance /sensitive genotypes; 2) construct the population for association analysis, genotyping by GBS-SNP and LD analysis; 3)determine and clone the major gene associated with heavy metal hexavalent chromium stress by combining genome-wide association (GWAS) analysis and RNA-seq. The results of the project will provide valuable basis materials and technical support for future evaluation and utilization of M. sinensis resource contributing to molecular breeding.