Non-coding RNA（ncRNA）是指不编码蛋白质的RNA，在原核细胞中通常称为sRNA。sRNA在细胞生命过程中起着十分重要的作用。在前期对于类囊体膜形成蛋白Thf1功能系统研究（Mol Microbiol 2016）的基础上，项目提出以光合作用模式生物蓝细菌Synechocystis sp. PCC 6803为材料，以我们新发现的ncRNA ThfR为研究对象，通过构建相应的过表达和敲降突变株，利用分子遗传、生理和生化等实验手段，研究ThfR与其靶标thf1的相互作用、调控机制及其结构基础，进一步研究对其相应蛋白Thf1功能的影响；通过建立的大肠杆菌表达平台模拟ThfR与thf1的表达，进一步研究ThfR的合成及其与thf1的相互作用机理。以期通过与其它光合作用相关ncRNA的研究结合，揭示光合作用这一地球上最重要反应的ncRNA调控网络，阐明其调控机制。

A non-coding RNA (ncRNA) is an RNA molecule that is not translated into a protein, often referred to as sRNA in prokaryotes, which plays very important regulatory roles throughout the whole life cycle of cells. Photosynthesis is a process used by plants and other organisms to convert light energy into chemical energy, the carbohydrate molecules, which are synthesized from carbon dioxide and water. Photosynthesis maintains atmospheric oxygen levels and supplies all of the organic compounds and most of the energy necessary for life on Earth, and was considered the most important chemical reaction on the planet. In the current proposal, by using the model cyanobacteria Synechocystis sp.PCC 6803, which has been used extensively for studies concerned with photosynthesis, we proposed to study the mechanism of action of ThfR, the newly discovered sRNA in our lab, with its target gene - the thylakoid formation 1 (thf1), on the basis of our earlier study of Thf1 (Mol Microbiol 2016). The interaction, regulatory mechanism as well as the structural basis of ThfR and its target thf1 will be elucidated, with the in-depth study of the overexpressor and surppressor mutant strains, through a series of methodologies including molecular genetics, physiology and biochemistry. Together with the discovery and studies of other ncRNAs related to photosynthesis, it is hoped to be able to reveal the ncRNA regulatory network of the world’s most important reaction.