移植后神经干细胞（NSC）的存活、增殖、分化以及与宿主神经网络的整合是影响NSC移植治疗效果的关键因素。最新研究表明缝隙连接蛋白Connexin 43（Cx43）介导的细胞间通讯连接（GJIC）参与移植细胞和宿主细胞之间的离子和代谢偶联，且Cx43对NSC的增殖，迁移和分化具有调控作用。本项目基于脑出血后Cx43表达变化的前期研究基础，探讨1）GJIC是否参与NSC与宿主细胞的整合并维持NSC的存活；2）脑出血环境下，GJIC的建立对星型胶质细胞Cx43的表达以及胶质细胞活化的影响；3）通过调控NSC中Cx43及Cx26的表达，诱导NSC向神经元定向分化；4）在脑出血模型中观察条件性控制移植NSC的Cx43和Cx26表达对移植NSC的存活及与宿主神经网络的整合能力以及小鼠神经功能改善情况。本研究通过调控NSC与宿主细胞的相互作用及定向分化，促进NSC移植的转化应用，为脑出血治疗提供新思路。

The survival, proliferation and differentiation of neural stem cells (NSCs) after transplantation and the integration with host neural networks are key factors affecting the therapeutic effect of NSCs transplantation. Recent studies have shown that connexin 43 (Cx43) -mediated gap junctional intercellular communication (GJIC) is involved in the ion and metabolic coupling between transplanted cells and host cells. Also Cx43 is a regulator of the proliferation, migration and differentiation of NSCs. Based on the preliminary finding of Cx43 expression after intracerebral hemorrhage, we come up with this proposal. We will explore whether the survival of NSCs and the integration of NSCs with host cells depend on GJIC and whether the establishment of GJIC helps to prevent Cx43 downregulation in astrocyte under the hemorrhagic environment. Driving NSCs to differentiate into neurons by regulating the expression of Cx43 and Cx26 in transplanted NSC. Neural network integration ability and neurological function improvement will be observed in the intracerebral hemorrhage model treated with NSCs transplantation. This study promoted the transformation application of NSCs transplantation by regulating the interaction of NSCs with host cells and differentiation of NSCs. It will provided new strategy for the treatment of ICH.