饲喂硝酸钠及延胡索酸二钠调控水牛瘤胃甲烷生成对脂肪酸生物氢化途径的影响及相关微生物学分析

作为整个氢转移过程的一部分，瘤胃甲烷生成与脂肪酸生物氢化途径之间存在相互联系。然而，目前关于调控瘤胃甲烷生成对脂肪酸生物氢化途径的影响以及相关微生物变化的研究却十分薄弱。本研究拟采用体外法研究硝酸钠及延胡索酸二钠调控水牛瘤胃甲烷生成对脂肪酸生物氢化途径的影响规律；分析比较不同微生物菌群对瘤胃脂肪酸代谢的贡献大小；在此基础上，研究饲喂硝酸钠及延胡索酸二钠调控水牛瘤胃甲烷生成对水牛瘤胃发酵、泌乳性能、乳脂肪酸组成的影响，分析脂肪酸生物氢化途径相关关键菌群的结构组成以及数量变化，最终揭示利用硝酸钠及延胡索酸调控瘤胃甲烷生成过程中脂肪酸生物氢化途径以及相关微生物菌群结构的变化规律，为有效调控瘤胃甲烷生成、提高畜产品中不饱和脂肪酸，尤其是共轭亚油酸比例提供理论依据。

It is proposed that, as two parts of hydrogen transfer process, there is a close link between methanogenesis and fatty acid biohydrogenation. Interventions to manipulate methanogenesis in the rumen inevitably lead to effects on fatty acids metabolism, including fatty acids biohydrogenation. However, there is little known about the interaction between methanogenesis and fatty acids biohydrogenation and changes related microbial activities and population in the rumen. Therefore in this study, firstly, we will analyse effect of methanogenesis on fatty acids biohydrogenation in vitro by sodium nitrate and disodium fumarate; Secondly, the contribution to different parts of ruminal microflora to the biohydrogenation will be compared with each other; Thirdly, based on above results, effect of sodium nitrate and disodium fumarate on ruminal fermentation, milk production and composition, ruminal and milk fatty acids profiles in water buffaloes will be studied; Lastly, the population and microbiota composition of microbiota related to biohydrogenation will be investigated. This study will be helpful for the understanding of the interaction between methanogenesis, fatty acids biohydrogenation and microbial community in the rumen, then can beneficial to the methane reduction , improvement of unsaturated fatty acids, especially for conjugated linoleic acid.