类金刚石（diamond-like carbon, DLC）薄膜拥有优良的力学和生物学性能，具备广阔的生物应用前景，解决尚存在的薄膜可控制备及其生物安全性等问题是目前的研究重点，而从分子水平阐明DLC薄膜与细胞界面的生物学过程是解决上述问题的关键。本项目通过元素掺杂、表面功能化等手段调控制备具有不同结构特征的DLC薄膜，结合石墨烯和纳米金刚石薄膜对照组，采用高速延时摄像原子力显微镜和单颗粒电镜技术对薄膜表面吸附的典型血清蛋白的动态行为和三维结构进行表征，并研究人体成骨细胞在薄膜表面行为，从而揭示影响关键血清蛋白贴附和后续细胞行为的DLC薄膜关键结构和表面物理化学特性，最终建立典型碳纳米材料表面结构特征、贴附蛋白构象变化和细胞行为三者之间的系统关系，为DLC薄膜的结构设计与可控制备和薄膜表面细胞行为的调控提供基础数据。

Owing to its superior biological and mechanical properties, diamond-like carbon (DLC) film has emerged as promising layer for biomedical devices. Yet quality control and bio-safty of DLC films has been one of the major challenges limiting their extensive applications. Clarification at molecular level of the interactions between DLC film and cells is the key to solving the problems. It is virtually known that upon contact of cells or tissues with biomaterials, initial absorption and associating conformational changes of serum proteins takes place as the initial event particiating in the mateiral-cell interactions. This project aims to reveal the effect of the characteristics of the DLC films on dynamic adsorption behaviors of key serum proteins and subsequent cell behaviors. The DLC films are to be fabricated with controllable micro-/nano- structrues through element doping and surface functionalization. Both graphene films and nano-diamond films will also be produced for the investigation. The dynamic adsorption of the proteins will be monitored by high-speed time-lapse AFM and the single particle electron microscopy technique is used for reconstructing 3D structures of the proteins. This research is anticipated to give clear insights into design and fabrication of DLC films with appropriate performances for biomedical applications.