人细小病毒B19（B19病毒）是输血和血液制品安全中倍受关注的病原体。对B19病毒生物学特性的充分认识和深入研究，是保障输血安全以及血液制品安全的必要条件。本课题组在前期研究中发现我国献浆人群中存在B19病毒重组型和未知基因型，在此基础上，本课题拟对我国献浆人群中不同基因型/亚型B19病毒代表株进行全基因克隆，利用生物信息学手段研究我国B19病毒的分子生物学特性及其系统发育情况。同时，构建并鉴定不同基因型/亚型B19病毒的感染性克隆，通过感染UT7/EPO细胞，在细胞模型上深入研究我国献浆人群中各基因型/亚型B19病毒代表株的生物学特性。本研究对于保障我国输血和血液制品的病毒安全性具有重要意义。

Human parvovirus B19 (B19V) has raised great concerns on viral safety of transfusion and blood products. Thus, in order to ensure the safety of transfusion and blood products, it’s quite necessary to do deep research on B19V’s biological characteristics. Our previous results indicated that there were intergenotypic recombinant B19V and unknown genotypes in Chinese plasma donors. In this project, we intend to construct and sequence the full-length genome clones of different genotypes/subtypes, then the sequences will be aligned with the B19V sequences retrieved from GenBank and phylogenetic trees will be constructed. Based on these results, infectious clones of different genotypes/subtypes B19V strains will be constructed and identified. These infectious clones will be used to infect UT7/EPO cells and further studies on the biological characteristics of B19V will be carried out. This study is significant for guaranteeing the safety of transfusion and blood products in China.