减数分裂同源重组过程中，交叉结形成是极为复杂而又关键的步骤，充分认识这一复杂的生物学过程，了解其作用网络与调控路径,将有助于实现对它的遗传调控。ZHIP1是我们利用已经获得的减数分裂同源重组相关蛋白ZIP4和HEI10等筛选水稻cDNA文库，并进一步从水稻减数分裂突变体库中克隆的一个与交叉结形成相关的新基因。本项目拟在此基础上，通过分子细胞学、遗传学以及生物化学等方法对ZHIP1的生物学功能进行深入研究，了解ZHIP1对交叉结数目和联会复合体形成调控的分子机理，明确ZHIP1在重组频率调控中的重要功能，探索水稻重组频率遗传调控的有效途径与方法。通过本项目的实施，预期结果不仅能够加深我们对减数分裂交叉结形成机理的理解，同时也为ZHIP1在水稻育种中的应用提供理论指导。

During homologous recombination,the formation of chiasma is a very complex and important step.Therefore,fully understanding this complex biological process and its regulatary pathway will facilitate the achievment of genetic regulation of chiasma.In our previous study, ZHIP1 was isolated and identified through screening the rice cDNA library and meiotic mutant library.ZHIP1 was found to be a novel gene required for the formation of chiasma. In this proposal,we will focus on the following aspects:①to further study the biological function of ZHIP1 by means of mocecular cytology,genetics,and biochemistry;②to unravel the regulation machanisms of ZHIP1 for the formation of chiasma and SC; ③to elucidate the role of ZHIP1 in the regulation of recombination frequency, and explore the effective way to genetically control recombination.The expected results will not only give us the deeper understanding of the molecular mechanism of the chiasma formation, but also provide theoretical guides for the application of ZHIP1 in the practical breeding.