肺癌作为死亡率极高的恶性肿瘤疾病，发展有效的标志物检测技术对于患者的诊断和治疗非常关键。微小核糖核酸（miRNA）因涉及肺癌的多个阶段而吸引了研究者的广泛关注。由于miRNA具有序列短，含量低等特点，现有的检测方法例如微阵列等技术在检测灵敏度和特异性方面都难以满足要求。石墨烯凭借其优异的导热和导电性能以及良好的表面可修饰性而被广泛的应用于传感器的设计。本项目拟发展基于石墨烯光热传感的检测技术，并将该技术与微流控结合起来用于肺癌标志物miRNA的检测。该技术利用石墨烯的偏振吸收光学性质并引入经过强度调制的泵浦光，通过锁相放大和平衡探测等弱信号检测技术实现对肺癌标志物miRNA的高灵敏度检测。基于石墨烯的光热传感技术具有免标记、高灵敏度以及微体积检测的优点。该技术不仅为肺癌标志物miRNA的高灵敏度检测提供新的思路，而且也为其它种类的疾病标志物和RNA类病毒的检测奠定一定的理论和实验基础。

In view of the fact that lung cancer is a malignant tumor disease with a very high mortality rate, the development of effective lung cancer biomarkers detection technology is critical for the diagnosis and treatment of patients. MicroRNA (miRNA) has attracted the attention of researchers because it involves multiple stages of lung cancer. However, due to the short sequence and low concentration of miRNA, existing detection methods, such as microarray, are difficult to meet the requirements in terms of sensitivity and specificity. Graphene is widely used in design of sensor due to its excellent thermal conductivity, electrical conductivity and good surface modifiability. In this project, the detection method based on graphene photothermal sensing will be developed. The technique is combined with microfluidics for the detection of lung cancer markers miRNA. The polarization-dependent absorption property of graphene and a modulated pump beam are utilized in this technique. The high sensitivity detection of lung cancer markers miRNA is achieved through weak signal detection techniques such as lock-in amplifier and balanced detector. Graphene photothermal sensing has the advantages of label-free, high sensitivity and micro-volume detection. This technique not only provides new ideas for high-sensitivity detection of lung cancer markers miRNA, but also lays a certain theoretical and experimental basis for the detection of other types of disease markers and RNA viruses.