PPR家族蛋白CSR3调控拟南芥叶绿体RNA剪接的分子机理
结题报告
批准号:
32000184
项目类别:
青年科学基金项目
资助金额:
24.0 万元
负责人:
张毅
依托单位:
学科分类:
植物光合与固氮
结题年份:
2023
批准年份:
2020
项目状态:
已结题
项目参与者:
张毅
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中文摘要
RNA剪接是指从新转录产生的前体RNA中去除内含子,并将相邻的外显子连接起来的过程,保证mRNA携带正确的遗传信息。RNA剪接是叶绿体基因表达与调控的关键环节,对叶绿体光合作用、代谢平衡和信号转导都有重要调节作用。陆生植物叶绿体内含子源于能够自我剪接的核酶,由于结构的变异,叶绿体内含子丧失了自我剪接能力,需要核编码蛋白协助其完成剪接过程。目前已经发现一些剪接因子,但叶绿体RNA剪接的分子机制还不清楚。我们筛选到一个拟南芥叶绿体RNA剪接缺陷突变体csr3(chloroplast splicing regulator 3),已有结果表明CSR3参与调控4个叶绿体光合作用关键基因内含子(ycf3内含子1、petB、ndhA和ndhB)剪接。本项目将以csr3突变体为研究材料,综合运用遗传学、生物化学等手段解析CSR3是如何调控叶绿体RNA剪接的,为揭示叶绿体RNA剪接分子机制提供新的认识。
英文摘要
RNA splicing represents an essential step for chloroplast gene expression, which removes the intron sequences of genes from pre-mRNA and joins the protein-coding sequences together to enable production of mature mRNA with correct genetic information. RNA splicing plays important roles in regulating photosynthesis, metabolic balance, and signaling of chloroplasts. Chloroplast introns of land plants derive from self-splicing ribozymes; however, they lost the ability to self-splice and require various nucleus-encoded proteins as cofactors. Although some nucleus-encoded splicing factors have been found, the molecular mechanism underlying chloroplast RNA splicing is still unknown. Recently, we identified a novel Arabidopsis chloroplast RNA splicing deficient mutant csr3 (chloroplast splicing regulator 3). We found that CSR3, encoding a PPR family protein, is required for splicing of ycf3 intron 1, petB, ndhA, and ndhB, which are all key genes for photosynthesis. This project aims to characterize the roles of CSR3 in chloroplast RNA splicing. Furthermore, it will provide new insights into molecular mechanism of chloroplast RNA splicing.
期刊论文列表
专著列表
科研奖励列表
会议论文列表
专利列表
DOI:10.1093/jxb/erad375
发表时间:2023
期刊:Journal of Experimental Botany
影响因子:--
作者:Mingyang Ma;Tong Zhu;Xiuyue Cheng;Mengyu Li;Guoliang Yuan;Changbao Li;Aihong Zhang;Congming Lu;Ying Fang;Yi Zhang
通讯作者:Yi Zhang
DOI:10.1016/j.xplc.2023.100611
发表时间:2023-09-11
期刊:Plant communications
影响因子:10.5
作者:Zhang Y;Tian L;Lu C
通讯作者:Lu C
国内基金
海外基金