基于宏基因组学16srDNA测序对云南地区中耳炎性疾病微生态环境分析及益生菌进行炎症干预的可行性研究

化脓性中耳炎其反复感染或久治不愈，一直是耳鼻咽喉科临床工作者感到棘手的问题。常见致病菌可能存在地区差异性，云南地区经济及卫生条件相对落后，中耳炎患者就诊时往往病史长，感染较重，且该地区目前对中耳炎性疾病病原学方面尚无相关研究报道。本研究对中耳炎患者的病原微生物行细菌培养，分析优势菌群，再利用宏基组学的16SrDNA测序法直接对细菌的16SrDNA基因进行扩增后测序。试图发现中耳炎性疾病微生态即菌群之间的相互关系，并以此建立云南地区中耳炎性疾病的微生态环境分析的理论基础。其次是将在肠道疾病及妇科炎症性疾病方面具有理想疗效及重要意义的益生菌微生态平衡平疗法的思想首次提出用于化脓性中耳炎的治疗理念中，并在建立的中耳炎感染的小鼠动物模型中进行益生菌与抗生素分别单独治疗及联合治疗的有效性的对比分析及验证。为中耳炎长期使用抗生素引起的耐药及迁延不愈提供一种新的健康，有效可行的治疗方法。

Suppurative otitis media is a common disease of otolaryngology, with the characteristic of repeated infection or prolonged treatment, becoming a thorny issue for otolaryngology clinicians. There is a huge difference in the common pathogenic bacteria of chronic suppurative otitis media. The economic and sanitation conditions in Yunnan are relatively backward, so the patients with otitis media often suffer long illness and the heavier infection. And no research about otitis media disease etiology in the region has been reported currently, therefore, we firstly cultivate bacteria of pathogenic microorganisms in patients with otitis media, analyze the dominant bacterial community, amplify and sequence bacterial 16S rDNA gene by using the argenomic 16S rDNA of Acer group learning directly, thus finding the relationship in the micro-ecology that relies solely on bacterial culture and can not be analyzed in the middle ear cavity microbiology ,namely flora. And then we establish the theoretical basis of micro-ecological environment of otitis media inflammatory disease in Yunnan Province. Secondly, we put forward the concept of probiotics micro-ecological balance therapy used in the treatment of suppurative otitis media for the first time, which has the desired therapeutic effect and significance on intestinal diseases and gynecological inflammatory diseases. Besides, probiotics and antibiotics during the established models of otitis media-infected mice Animal are treated separately and the effectiveness of the combination is compared and analyzed. At last, we provide a new and healthy, effective and feasible treatment method to treat otitis media patients who use antibiotics for a long time and cause drug resistance.