Bacterial diversity of middle ear cholesteatoma by 16S rRNA gene sequencing in China.
Bacterial diversity of middle ear cholesteatoma by 16S rRNA gene sequencing in China.
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DOI：
10.1007/s10142-023-01068-2
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发表时间：
2023-04-27
影响因子：
2.9
通讯作者：
Ruan, Biao
中科院分区：
文献类型：
--
作者：
Liang, Qiulin;Long, Ruiqing;Li, Shuling;Jiang, Chaowu;Gao, Jingyu;Cheng, Sheng;Liu, Zhuohui;Ruan, Biao
本研究通过对获得性中耳胆脂瘤（MEC）的细菌多样性进行评估，揭示其发病机制，并为抗生素的使用提供指导。对29例获得性中耳炎患者和8例行人工耳蜗植入术的健康中耳患者进行评价。采用全长度16S rRNA基因测序分析中耳病变组织和健康组织中的细菌群落。ACE指数（P = 0.043）和Chao1指数（P = 0.039）差异有统计学意义（P < 0.05）。使用Bray-Curtis距离矩阵对PERMANOVA/Anosim结果进行分析，组间差异大于组内差异（R = 0.238, P < 0.05, R2 = 0.066, P < 0.05）。细菌群落分析显示，甲变形菌属在纲水平上、茎杆菌属和鞘单胞菌属在目水平上差异显著（P < 0.05）。在LefSe （Linear discriminant analysis effect size）分析中，在MEC的物种水平上，bennonis卟啉单胞菌（Porphyromonas）升高，而Bryum argenteum和未分类蓝藻（unclassified Cyanobacteriales）降低（P < 0.05）。通过分析《京都基因与基因组百科全书》（KEGG）第2级代谢途径丰度，发现两组间有15条代谢途径存在显著差异。MEC组和对照组分别显著升高了7条和8条代谢途径（P < 0.05）。通过对代谢途径的分析，进一步明确了细菌在获得性MEC发病机制中的作用。这些发现表明，获得的MEC和健康的中耳含有比以前认为的更多样化的微生物群落。在线版本包含补充材料，可在10.1007/s10142-023-01068-2获得。
In this study, the bacterial diversity of acquired middle ear cholesteatoma (MEC) was evaluated to reveal its pathogenesis and provides a guide for the use of antibiotics. Twenty-nine cases of acquired MEC and eight cases of healthy middle ears undergoing cochlear implantation (CI) were evaluated. Full-length 16S rRNA gene sequencing was performed to profile the bacterial communities in lesions and healthy tissues of the middle ear. ACE (P = 0.043) and Chao1 (P = 0.039) indices showed significant differences in alpha diversity (P < 0.05). Analysis of PERMANOVA/Anosim using the Bray–Curtis distance matrix results suggested that the between-group differences were greater than the within-group differences (R = 0.238, P < 0.05, R2 = 0.066, P < 0.05). Bacterial community analysis revealed that Alphaproteobacteria at the class level and Caulobacterales and Sphingomonadales at the order level were significantly different (P < 0.05). In the LefSe (Linear discriminant analysis effect size) analysis, Porphyromonas bennonis was elevated, and Bryum argenteum and unclassified Cyanobacteriales were reduced at the species level in MEC (P < 0.05). Fifteen metabolic pathways were found to be significantly different between the two groups by analysing the abundance of metabolic pathways in level 2 of the Kyoto Encyclopaedia of Genes and Genomes (KEGG). Seven and eight metabolic pathways were significantly elevated in the MEC and control groups, respectively (P < 0.05). The role of bacteria in the pathogenesis of acquired MEC was further refined through analysis of metabolic pathways. These findings indicate that the acquired MEC and healthy middle ear contain more diverse microbial communities than previously thought. The online version contains supplementary material available at 10.1007/s10142-023-01068-2.
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