快速构建稳定、高效的菌-藻共生好氧颗粒污泥体系，是推进菌-藻共生污水处理系统发展的关键与难点。本项目基于对微藻富集条件及菌-藻聚集行为的研究，明确菌-藻共生好氧颗粒污泥的形成及成长机制，并提出分段控制策略，以探明菌-藻共生好氧颗粒污泥体系构建的工艺原理；应用分子生物学手段，考察污泥颗粒化进程中菌-藻共营生态系统在时间和空间维度下的演替规律并构建菌-藻分子生态网络；分析菌-藻生态演替对共生体聚集行为、结构稳态及功能特性的影响，揭示其在污泥颗粒化进程中的作用机制。基于以上研究，识别影响共生颗粒污泥中菌-藻群落结构的主导因素，并判明两者间的映射关系，最终以系统的构建效率、结构稳态及功能特性为评价指标，提出基于生态调控的菌-藻共生好氧颗粒污泥体系强化构建策略。研究成果将为菌-藻共生体颗粒化技术的工程化应用提供必要的理论基础与技术支撑。

Rapid construction of stable and efficient bacterial-algal symbiotic aerobic granule system is the key and difficult point to promote the development of bacterial-algal symbiotic wastewater treatment system. In this project, based on the analysis of microalgae enrichment conditions and aggregation behavior between bacteria and algae, the formation and growth mechanism of bacterial-algal symbiotic aerobic granules will be clarified. Furthermore, the piecewise controlling strategy will also be proposed to ascertain the construction process principle of bacterial-algal symbiotic aerobic granules system. Molecular biology methods will be adopted to investigate the spatial-temporal succession of bacterial-algal symbiotic ecosystem during the sludge granulation process, as well as the molecular ecological network of bacterial-algal consortia will be constructed. By analyzing its influence on aggregation behavior, structural stability and functional performance of bacterial-algal symbiotic granules, the action mechanism of bacterial-algal ecological shift on the granulation process will be revealed. In this case, the dominate factors influencing the bacterial-algal community in symbiotic aerobic granules will be identified and the relationship between them will also be determined. According to the evaluation of construction efficiency, structural stability and functional performance, the strengthening construction strategy for bacterial-algal symbiotic aerobic granules system which is based on the ecological regulation will be proposed. The research results will provide necessary theoretical basis and technical support for the engineering application of bacterial-algal symbiotic aerobic granules.