基于代谢组和转录组联合分析的咖啡酸与IAA对牡丹试管苗不定根发生机制研究

牡丹试管苗根系发生困难、不定根与茎的输导组织不连接、移栽成活率低等问题至今未能得到有效解决。因此，开展牡丹试管苗不定根发生代谢和分子机理研究，应是解决该难题可能途径之一。前期研究发现，酚类物质与牡丹试管苗生根密切相关，二元酚和IAA配合使用能显著提高其生根率，以添加咖啡酸生根率最高，但咖啡酸与IAA促进不定根发生的作用机理尚不清晰。因此，本研究以牡丹试管苗‘凤丹白’为研究对象，运用代谢组和转录组技术，分别从代谢物水平和RNA转录水平对不定根发生不同阶段的代谢物和基因进行研究，建立与生根相关的代谢组和转录组数据库；利用代谢组和转录组的关联分析，综合探讨咖啡酸与IAA对牡丹试管苗不定根发生的作用机制；结合生理数据，探讨咖啡酸与IAA参与不定根发生表达调控的主要生物合成路径，挖掘与生根相关的候选基因，并深入解析相关功能基因，以期为建立牡丹高效植株再生体系提供理论和技术支撑。

Some problems have unable to effectively solved on tree Peony in vitro, such as rooting difficulty, low root quality, adventitious roots and transfusion tissue of stem cannot connect, low survival rate of transplanting. Therefore, one of the possible ways to solve this problem is to study the metabolism and molecular mechanism of adventitious root formation. It was found that phenols are closely related to the rooting of peony in vitro. The combination of diphenols and IAA can significantly improve the rooting rate, and caffeic acid has the highest rooting rate, but the mechanism of caffeic acid and IAA to promote adventitious rooting is not clear. Therefore, this study took 'fengdanbai' as the research object, and used metabolome and transcription technology to study metabolites and genes at different stages of adventitious rooting from metabolite level and RNA transcription level, respectively, and established the database of metabonomics and transcriptomics related to adventitious rooting. By using the correlation analysis of metabonomics and transcriptomics, we comprehensively discussed the effect of caffeic acid and IAA on the growth of tree peony in vitro. Based on the physiological data, the main biosynthetic pathways of caffeic acid and IAA involved in the regulation of rooting expression were explored, candidate genes related to adventitious rooting were mined, and the related functional genes were analyzed in order to provide theoretical and technical support for the establishment of efficient plant regeneration system of peony.