K 型细胞质温敏雄性不育小麦两用系A3017农艺性状优良，适应性广泛，在国内多个麦区正季播种完全雄性不育、反季播种稳定可育。系谱和遗传分析表明，A3017所携带的K型温敏恢复基因来源于斯卑儿脱小麦IBS染色体。本项目将利用SSR、AFLP和EST标记对该基因精细定位，为该基因的图位克隆奠定基础。同时对从A3017育性转换相关基因差异表达SSH-cDNA文库中获得的特异上调表达TaMS-MADSbox（大约660bp）和TaG3BP（大约1230bp）进行系统研究，采用Real-Time PCR技术分析其在小麦育性转换过程中的时空表达模式；利用染色质免疫共沉淀技术，查明受该基因调控的靶标基因；其次构建其过表达载体，干预在不同育性条件下该温敏材料的花发育进程，进而剖析其在育性转换过程中的生物学功能。该研究结果将丰富小麦雄性不育相关分子生物学理论，为小麦杂种优势利用和分子育种提供理论依据。

Temperature sensitive cytoplasmic male sterile line A3017, with Ae.Kotschyi cytoplasm，excellent agronomic traits, wide adaptability, showed completely male sterility in normal wheat production conditions as would be needed for producing hybrid seed, but partial fertility restoration in other seasons and growing conditions, thus producing hybrid parent seed. Pedigree and genetic analysis showed that the A3017 carried K-type thermo-sensitive resoration gene from T.spelta var. duh. wheat IB chromosome segment. The thermo-sensitive gene will be carried out fine mapping by the SSR, AFLP and EST markers, and the experimental result will provide condition for gene positional cloning. In addition,Fertility conversion related genes, specific upregulated TaMS-MADSbox (about 660bp) and TaG3BP (about 1230bp) genes from the SSH-cDNA library , will be studied as following: 1)using Real-Time PCR technology, fertility temporal and spatial expression patterns will be analyzed in the fertility conversion process; 2)By chromatin co-immunoprecipitation techniques, to identify target genes regulated by the gene; 3)Building its over-expression vector, and interfering with the flower developmental process of the temperature sensitive material in different fertility conditions, thereby analyze the biological functions during the fertility conversion process. The study results will be rich the molecular biological theory on wheat male sterility, and provide a theoretical basis for the utilization of wheat hybrid heterosis and molecular breeding.