生姜（Ginger）是多年生宿根草本植物姜的根状块茎，其药食两用的特点具有很高的经济利用价值。土壤高湿度下，生姜易染青枯菌是引起生姜大幅减产的主要原因，导致生姜植株枯死，块茎腐烂。探明土壤高湿度下生姜对青枯菌的应答机制，解析其关键基因作用机制，对开发生姜栽培技术避免姜瘟病暴发具有理论和实践意义。. 申请者在生姜大田栽培经验的基础上，拟进一步对不同土壤湿度下青枯菌致病力进行精准化分析，探索土壤高湿度下生姜易染青枯菌的生理生化机制；分析生姜转录组响应土壤湿度的表达差异，发掘参与响应青枯菌侵染的病程相关基因；克隆2-3个激素信号转导通路关键基因，采用酵母双杂交系统、转化模式植物等手段研究该基因在响应青枯菌侵染过程中的作用，重点从植物内源激素层面探讨生姜在土壤高湿度下响应青枯菌侵染的分子机制，为开发针对生姜的栽培技术提供依据，为生姜基因工程提供基因储备。

Ginger, is the rhizome of the perennial herb plant Zingiber officinale, with high economic value for the characteristics of both drug and food usage. Under high soil humidity, Rastonia solanacearum is more prone to infect the ginger, which could lead to plant die and rhizomes rot. And that’s the main cause of severe ginger reduction. Understanding the mechanisms of ginger responding to R.solanacearum, and the functions of some pathogenesis related genes under high soil humidity will provide the theoretical and practical significances for the development of ginger cultivation technology, and avoidance of the outbreak of ginger bacterial wilt.. On the basis of the cultivation experience of ginger, this study designed to explore the physiological and biochemical mechanism of ginger responding to R.solanacearum by analyzing the virulence of R. solanacearum; analyze the differential expression of transcriptome and screen the pathogenesis related genes involved in the response to R.solanacearum under high soil humidity; clone 2-3 key genes involved in hormone signal transduction pathway and use yeast two hybrid system and model plant to study their antibacterial function. This study is mainly from the plant endogenous hormone pathway to understand the molecular mechanism of ginger response to the infection of R.solanacearum under high soil humidity, and could provide basis of the exploration of ginger cultivation techniques, and candidate genes for ginger genetic engineering.