笋芽萌发是竹笋品质及材质形成的重要发育阶段，这一过程细胞壁果胶类多糖累积是笋快速高生长的主要物质基础之一。脱氧辛糖酸-8-磷酸合酶（KDO8PS）负责果胶中八碳糖KDO的生物合成，可调控不同组织器官的生长发育。申请者在前期研究工作中首次发现，酶寡聚体中同源二聚体和四聚体的相互转化同酶浓度变化正相关，结合该基因时空差异表达特征，推断在笋芽萌发过程中，寡聚体构象转变可通过酶结构稳定性的改变维持酶活性的不同状态，从而调控笋芽细胞壁多糖合成代谢。为探明KDO8PS同源寡聚体成因，理解其对于笋芽萌发的调控作用，本研究拟采用分子生物学及结构生物学等实验手段，在毛竹KDO8PS表达及分离纯化基础上，完成寡聚体结构解析；利用基因敲除技术、转基因过量表达及RNA-seq等技术，探讨该酶在笋芽萌发中的功能和作用，从而为今后揭示笋芽发育及材质形成的分子机制打下基础。本项目已获得拟南芥KDO8PS晶体结构。

Bamboo bud germination is an important development stage to form bamboo quality and material,which the accumulation of polysaccharide pectin class on cell wall is one of the main material in the fast growing shoots.3-Deoxy-D-manno-octulosonate-8-phosphate synthase(KDO8PS) is responsible for the bio-synthesis of eight carbon sugar KDO in pectin and thus regulates the growth and development of different tissues and organs.We first discovered in our previous research work that KDO8PS is a homo-oligomeric enzyme with two different protein conformation. KDO8PS from plant is demric and can be converted it to a tetramer in high concentration of enzyme solution. Combined with features of KDO8PS differential space-time gene expression, we inference that KDO8PS could change of the enzyme structure stability or maintain enzyme activity through oligomers conformation transformation and affect the cell wall polysaccharide biosynthesis process of bamboo shoots .For identifying the cause of KDO8PS enzyme oligomers to further understand its regulating role for bamboo shoot germination, in this study we will complete determination of KDO8PS enzyme structure with molecular biology and structural biology methods based on the heterologous expression and purification of bamboo KDO8PS. we also explore target enzyme functions and roles by using gene knockout technology, transgenic technology and RNA-seq analysis so that we can lay the foundation for revealing the molecular mechanism of bamboo shoot development and material formation in the future.The crystal structure of AtKDO8PS has been obtained.