枇杷果肉木质化形成过程的拉曼/红外定量显微可视化研究

木质化主要是木质素在细胞壁沉积的过程。常规枇杷果肉木质素检测方法多在组织水平获得木质素总含量，对于枇杷果肉木质化在细胞水平，特别是单细胞水平的形成和发展机制还不明确。本项目拟采用拉曼/红外显微高光谱成像技术结合数学分析算法，通过开展光谱响应分析、特征提取、光谱多尺度融合和指纹信息挖掘等工作，在保留细胞壁微观空间结构的基础上，构建木质素在枇杷果肉细胞壁上的非标记定量微观可视化体系，明晰不同采后处理下枇杷果肉木质化过程中木质素在细胞壁上的含量/分布动态变化。项目研究旨在为深入探讨枇杷果肉木质化机制提供理论和技术支撑。

Lignification is a process during which the lignin is depositing in cell walls. The lignin measurement of loquat fruit is usually carried out at the tissue level, result in a total lignin content. The formation and development mechanism of lignification of loquat flesh at the cell level, especially at the single cell level, is not clear yet. The present project will use Raman/IR microscopic hyperspectral imaging techniques in tandem with mathematical analysis algorithms to establish a label-free quantitative and microscopic visualization system for displaying the lignin distribution within the cell walls of loquat flesh. The system will be established through a series mathematical works including spectral response analysis, feature extraction, spectral multi-scale fusion and fingerprint information mining. The operation of this system can retain the micro-space structure of cell walls, and therefore will be further used to understand the dynamic change of content/distribution of lignin within the cell walls of loquat flesh processed by different postharvest methods during the lignification process. The results of the present project will provide theoretical and technical supports on further exploring the lignification mechanism of loquat flesh.