翅型分化是昆虫对不良栖息环境的适应，昆虫通过提高飞行能力逃离不良环境并寻获新的栖息地。然而，miRNA作为重要的后转录调控因子，是否介导生物胁迫因子诱导的昆虫翅型分化及其调控机制仍不明确。本项目以柑桔衰退病毒的主要传播媒介褐色桔蚜Aphis citricidus为研究对象，紧密围绕miR-9b调控生物胁迫因子诱导的褐色桔蚜翅型分化的分子机制这一关键科学问题，系统运用原位杂交、双荧光素酶报告系统、RNA干扰等技术明确褐色桔蚜miR-9b对ABCG4的靶向调控机制、解析miR-9b响应生物胁迫因子的表达模式、阐明miR-9b对胰岛素信号通路活性及糖脂代谢的调控和对翅型分化的影响，最终揭示miR-9b调控褐色桔蚜翅型分化的分子机制。研究结果不仅有助于解析翅二型昆虫翅型分化调控机理，还有利于寻获新的小分子RNA控制剂靶标，为蚜虫类害虫防控提供新的思路。因此，具有重要的理论意义和应用前景。

Wing dimorphism functions as an adaptive switch to environmental changes that winged morphs obtained the flight capability to escape unfavorable conditions and to migrate to new suitable habitats. However, as a key critical component of post-transcriptional gene expression regulation, whether miRNA involved in biotic stressors mediated wing dimorphism and the molecular mechanism is still unclear. Here, The main vector of Citrus tristeza virus, Aphis citricidus, is used to focus on the molecular mechanism of miR-9b regulated various biotic stressors-mediated wing dimorphism of the aphid. Many technologies such as in situ hybridization, Dual-Glo luciferase report system and RNA interference are using to point out the targeted relationship between miR-9b and ABCG4; the expression patterns of miR-9b in response to various biotic stressors is investigated; the mechanism of miR-9b mediated activating of insulin pathway and glucose/lipid metabolism are analyzing and finally clarifying the molecular mechanism of miR-9b regulated wing dimorphism in A. citricidus. The results are useful to understand the wing dimorphism of insects and also promote to find the novel small RNA target as an insecticide as well as provide a new control strategy on the aphid pests. Therefore, it has important theoretical significance and application prospects.