黄曲霉是人和动物的条件致病菌，项目前期从黄曲霉中分离得到病毒AfPV1，并携带卫星RNA，AfPV1能够引起寄主一系列的衰退症状，而卫星RNA能够缓解AfPV1单独侵染造成的症状，并且影响AfPV1在寄主细胞内的复制和累积，但目前对于卫星RNA在丝状真菌中的具体功能并不清楚。因此本项目拟通过AfPV1和卫星RNA的水平传染，观测卫星RNA对AfPV1及寄主的影响；采用qRT-PCR、Northern blot和Western blot分析卫星RNA对AfPV1的表达量和积累量的影响；应用Solexa和生物信息学技术进行筛选，采用qRT-PCR、基因敲除等技术进行验证，得到黄曲霉调控AfPV1、卫星RNA感染的基因通路。本项目将在黄曲霉中建立真菌病毒、卫星RNA及寄主互作系统，阐明真菌病毒中卫星RNA的功能和真菌调控病毒、卫星RNA感染的基因通路，同时为深入研究三者互作机理奠定基础。

Aspergillus flavus is an opportunistic pathogen of human and animal. In previous study, a novel mycovirus AfPV1 with a satellite RNA was isolated from A. flavus strains. AfPV1 infection result in debilitation symptoms for its host, but the satellite RNA can alleviate these symptoms and also affect replication and accumulation of AfPV1. As we know, the biological function of satellite RNA is not clear in filamentous fungi. The effect of satellite RNA on AfPV1 and its host will be detected by transfecting AfPV1 and satellite RNA. The effect of satellite RNA on the expression and accumulation of AfPV1 will be analyzed by using qRT-PCR, Northern blot and Western blot. The gene pathways for A. flavus to regulate AfPV1 and satellite RNA infection will be screened out using Solexa and bioinformatics technology, and then verified by qRT-PCR and gene knockout technology. This project will establish the interaction system among satellite RNA, mycoviruses and host in A. flavus, clarify the function of satellite RNA in fungal virus and the gene pathways for regulations of viral and satellite RNA infection in fungal, and lay the foundation for in-depth study of the interaction mechanism of the three.