黔西北大面积历史遗留铅锌废渣堆场重金属的持续释放引起的周边水-土环境污染问题备受关注。植被重建是减少废渣堆场重金属迁移扩散的重要生态措施，当前研究主要关注植物根际环境中溶解性有机质（DOM）对废渣重金属迁移转化的影响。植物根际环境中DOM与微生物间存在交互作用，然而目前关于DOM-微生物耦合对废渣重金属迁移转化的影响机理尚不清楚。本项目拟以黔西北已开展生态修复多年的历史遗留铅锌废渣堆场为研究对象，应用微宇宙培养、元素地球化学、矿物学、光谱、质谱及分子生物学等方法，研究不同来源DOM-微生物-废渣耦合系统中DOM组分与微生物群落动态变化及废渣重金属迁移转化特征，阐明DOM组分变化-微生物群落演替-废渣重金属迁移转化特征间的耦合关系，识别影响废渣重金属动态的关键DOM组分与微生物群落，揭示DOM-微生物耦合对废渣重金属迁移转化的影响机理，为在金属冶炼废渣堆场上科学开展生态修复工程提供理论支撑。

The continues release of heavy metals from the large area of historical legacy of Pb-Zn slag site in northwest Guizhou has caused serious heavy metals pollution to the surrounding water-soil environment. The establishment of vegetation is an important ecological measure to reduce the diffusion of heavy metals in the waste slag site. The recent studies focus on the effect of dissolved organic matter (DOM) on the transportation and transformation of heavy metals in the rhizosphere environment of plant. There is an interaction between DOM and microorganisms in the plant rhizosphere environment, however, the coupling effects of DOM-microorganisms on the transportation and transformation of heavy metals in the Pb-Zn waste slag is still unclear. The revegetated historical legacy of Pb-Zn waste slag site in northwest Guizhou as the subject in the present proposal. The dynamic changes of DOM components and microbial communities and the transportation and transformation characteristics of heavy metals in the coupling systems of DOM-microorganisms-waste slag will be explored using the methods such as the micro-cosmic culture, elemental geochemistry, mineralogy, spectroscopy, mass spectrometry, and molecular biology. The coupling relationships among the DOM components change, the microbial communities succession, and the transportation and transformation characteristics of heavy metals in the waste slag will be explained. The key DOM components and microbial communities that influencing the dynamics of heavy metals in the waste slag will be identified. Finally, the coupling effect mechanisms of DOM-microorganisms on the transportation and transformation characteristics of heavy metals in the waste slag will be revealed. The results obtained are expected to provide a theoretical support for scientifically conducting ecological restoration engineering in the metals smelting waste slag sites.