小麦MATE转运蛋白基因ITR参与株型调控的分子机制研究

批准号:
32001497
项目类别:
青年科学基金项目
资助金额:
24.0 万元
负责人:
邓清燕
依托单位:
学科分类:
作物种质资源学
结题年份:
2023
批准年份:
2020
项目状态:
已结题
项目参与者:
邓清燕
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中文摘要
作物高产育种离不开理想株型的建立。因此,发掘小麦株型基因、解析株型调控的分子机理,具有十分重要的理论意义和应用价值。植物株型是一个十分复杂的性状,涉及大量基因和几乎所有组织。在前期研究中,我们在二倍体小麦中克隆了一个参与分蘖数、株高、节间数和叶片数等性状调节的株型相关基因TmITR。该基因编码定位于反式高尔基体的MATE转运蛋白,具有12个预测的跨膜螺旋。其突变体表现为极度矮化、分蘖剧增、叶片和节间数目增加、叶片变窄。在普通小麦中表达该基因也导致株高降低和分蘖数增加。但是,小麦ITR参与株型调控的分子机制仍不清楚。本项目拟从表达谱、剂量效应、转录组变化、蛋白互作、作用途径等方面解析ITR发挥功能的机制,丰富小麦理想株型建立的理论基础。
英文摘要
Establishment of ideal plant architecture can significantly enhance yield potential of crops. Identification, cloning and characterization of genes related with plant architecture is therefore of great significance for deciphering the mechanisms underlying plant architecture regulation and crop improvement. As a complex trait, plant architecture involves a large number of genes and almost all tissues. We have isolated a plant architecture-related gene TmITR in diploid wheat, which encodes a trans-Golgi localized MATE transporter with 12 predicted transmembrane helices. TmITR mutation led to dwarfism, more but narrower leaves, and more tillers and internodes. Expressing TmITR in common wheat also resulted in reduced plant height and increased tiller number as well. However, how TmITR participates in plant architecture regulation is still unknown. In this study, we propose to characterize TmITR through gene expression, dosage effect, transcriptome profiling, protein-protein interactions and molecular network, in order to explore the molecular basis of plant architecture formation.
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