胡蔓藤内生真菌抗肿瘤新结构次生代谢产物研究

抗肿瘤药物研发具有紧迫性和重要性。药用植物胡蔓藤含有种类丰富的化学成分，具有广谱生物活性，而根据内生真菌与宿主植物长期协同进化的互相影响，极有可能从胡蔓藤内生真菌中分离到优活性、新结构的化合物。前期我们从该类真菌中发现部分菌株具有抗肿瘤活性。本研究以该类真菌为研究对象，结合种属排重、化学排重、活性评估优选菌株，利用HPLC-DAD-HRMS液质快速检识目标化合物，实现新结构活性化合物的定向分离和富集。本项目旨在寻找具有抗肿瘤活性的新结构化合物，为挖掘抗病微生物菌种、开发创制新型抗肿瘤药物以及促进胡蔓藤内生真菌资源的开发和合理利用提供理论和实验依据。

The research and development of antitumor drug are urgent and important. Based on the interaction of long-term coevolution between endophytic fungi and host plants, it is most likely that the compounds with excellent activity and new structure are able to be isolated from the endophytic fungi of Gelsemium elegans Benth. In our previous study，we found that some of these fungi had antitumor activity. This proposal aims at the discovery of new antitumor secondary metabolites from the endophytic fungi of Gelsemium elegans Benth. by application of advanced technologies such as HPLC-DAD-HRMS. The results will lay the foundation for discovering new resistant strains and genetic resources, and promote exploitation and utilization of the endophytic fungi from Gelsemium elegans Benth.