外源性有害残留物的检测对中药质量评价具有重要的意义，有助于严格限制中药中污染物的残留量，进而确保病人的身体健康。为了解决已有检测方法在检测效率和特异性上的缺陷，本项目基于组分多样、结构可控以及易修饰的MOFs纳米材料，研究针对外源性有害残留物的新型化学发光免疫分析方法。具体内容包括：①将具有高效类酶活性（例如氧化酶、水解酶、还原酶）的MOFs作为信号探针，催化化学发光反应，构建MOFs纳米酶型免疫分析技术。②将高负载量的MOFs作为载体，高效载入化学发光体系的催化剂、发光剂（例如吖啶酯、鲁米诺），提高发光体系的发光信号，构建化学发光试剂-MOFs复合物型免疫分析方法。③将具备高氧化活性的MOFs作为增敏剂，显著地增敏化学发光体系信号，构建MOFs类芬顿试剂型免疫分析平台。本研究有望制备稳定性良好、发光性能优异的MOFs化学发光探针，并构建用于外源性有害残留物检测的免疫分析平台。

Development of detection protocols for extrinsic harmful contaminants in traditional Chinese medicines is of great significance for the insurance of drug safety. The reported protocols for the detection of these contaminants often suffer from low testing efficiency and unsatisfied selectivity. Various functional metal-organic frameworks (MOFs) have attracted increasing interest due to their unique physical and chemical properties, easy modification, exceptional tunability of structures and inner porosity. In the present project, MOFs will be utilized to conduct several chemiluminescent (CL) immunoassay approaches for the detection of harmful contaminants in traditional Chinese medicines. Firstly, some MOFs possessing high enzyme-like activity, such as peroxidase mimetics, hydrolase mimetics and reductase mimetics, will be adopted as CL probes for the development of enzyme-mimetic MOFs-based CL immunoassay. Secondly, by utilizing the encapsulating of CL reagents such as acridinium ester and luminol, MOFs with enormous pores will be used as probe carriers for the development of highly sensitive MOFs-based CL immunoassays. Thirdly, due to the increased CL signals by Fenton-like MOFs, MOFs will be utilized as CL enhancers in Fenton-like MOFs-based CL immunoassays. The project can achieve stable and reliable MOFs-based CL probes for the development of highly sensitive and selective immunoassay platform for the detection of extrinsic harmful contaminants in traditional Chinese medicines.