海洋微型真核浮游生物因其在微食物网内的重要功能而成为海洋、生态、环境等领域重要的研究对象。迄今的工作表明，人们对微型真核浮游生物群落多样性对水体环境状况的响应了解仍很粗浅。为此，本课题选择黄海近岸海域，围绕微型真核浮游生物群落多样性的时空分布特征，借助高通量测序手段和多元分析手段探讨其分类多样性参数分布变化规律，及对水体环境状态变化的响应机制，特别是确定主要的环境影响因素。本工作将微型真核浮游生物对不同季节、营养盐梯度等因素带来的环境差异的响应作为研究重点，填补此类研究的空白。本工作首次将群落生态、分子测序技术与分类多样性参数整合并据此探讨海洋微型生物对海洋近岸水体富营养等环境变化的响应机理，将为生物生态学、海洋环境学等研究提供重要的理论依据，为更好的理解近岸海洋环境变化对生物多样性的影响提供新的角度和基础数据。

Marine microbial plankton are the most abundant biological entities in marine ecosystems and play a crucial role in the functioning of microbial food web. In order to understand the response of microbial plankton diversity to environmental variations, we plan to study the community structure characteristics, genetic diversity, and distribution dynamics of the microbial eukaryotes by the high throughput sequencing and multivariate analyses in coastal waters of Yellow Sea which is particular stressed area and presents a unique marine ecosystem. Via analyzing the tempo-spatial variation patterns in genetic diversity and taxonomic diversity indices of microbial eukaryote community, we therefore expect to determine the forcing environmental factors which affect biodiversity most. It is the first time to combine community ecology, high throughput sequencing, and taxonomic diversity indices together to study marine microbial plankton diversity and their response to environmental changes. And, the results of this study will help us to understand the characteristics of microbial ecology and provide the foundational data for future ecological and environmental studies.