β-分泌酶（BACE1）是阿尔茨海默症（Alzhemier's disease, AD）药物治疗的一个重要靶标，其活性检测以及抑制剂的筛选对防治AD药物的研发至关重要。本项目拟利用BACE1特异性剪切多肽的性质构建一种GMR多肽传感器用于BACE1的灵敏检测。项目将通过制备新型磁标记材料——Ni-NTA@Fe3O4复合磁性纳米颗粒，设计新的磁标记方式和多肽探针，探索GMR传感器表面生物固定方式，优化GMR传感器的测试条件，从而实现GMR传感器对β-分泌酶的检测，在此基础上项目还将通过在GMR生物传感器芯片表面集成微流体通道来建立高通量筛选β-分泌酶抑制剂的新方法。研究结果将为β-分泌酶的灵敏检测以及其抑制剂的高通量筛选提供一个新平台，在阿尔茨海默症的诊断、治疗以及相关药物开发领域有广阔应用前景。

β-Secretase(BACE1) is regarded as an important drug target in the treatment of Alzhemier's disease(AD), therefore development of the method to detect BACE1 and screen its inhibitor represent an important step for the drug discovery. This project intends to construct a high sensitive GMR biosensor based on the target-induced cleavage specific peptide for detection of BACE1. Through preparing new magnetic labeling material, designing magnetic labeling mode and peptide probe, exploring the biological fixation of the sensor surface, and optimizing test condition of GMR sensor, the assay will be achieved. Furthermore, through integrating microfludic channel on the GMR biosensor, a novel method is also developed for screening of BACE1 inhibitors. This research work will provide a novel biosensing platform for detection of BACE1and screening of its inhibitors, which has a wide application prospect in the diagnosis of AD and drug discovery.